A Bacillus thuringiensis hlj-66 active against bt diamondback moth and its application

A technology of HLJ-66 and Bacillus aureus is applied to a strain of Bacillus thuringiensis HLJ-66 which is active against Bt diamondback moth and its application field, which can solve the problems of pest resistance that cannot be ignored, and achieve good application and promotion prospects. Improve economic value, the effect of strong poisoning effect

Active Publication Date: 2015-09-09
顶秀作物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, with its long-term use and the rapid promotion of Bt crops, the resistance of pests to Bt toxin has become a problem that cannot be ignored.

Method used

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  • A Bacillus thuringiensis hlj-66 active against bt diamondback moth and its application
  • A Bacillus thuringiensis hlj-66 active against bt diamondback moth and its application
  • A Bacillus thuringiensis hlj-66 active against bt diamondback moth and its application

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Experimental program
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specific Embodiment approach 1

[0013] Embodiment 1: A strain of Bacillus thuringiensis HLJ-66 active against Bt diamondback moth in this embodiment is Bacillus thuringiensis HLJ-66, which is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee , the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit date is November 8, 2013, and the deposit number is CGMCCNo.8445.

[0014] In order to achieve the above object, the present invention adopts the following technical measures:

[0015] A strain of Bacillus thuringiensis isolated from soil. Fermentation is carried out with fermentation medium, and cultured until paraspora crystals fall off. Bioassays were carried out on relatively sensitive Plutella xylostella, Btk-resistant Plutella xylostella and Cry1Ac-resistant insecticidal crystal protein Plutella xylostella, Tenebrio molitor, and all had strong insecticidal effects on the above-mentioned tested pests...

specific Embodiment approach 2

[0035] Embodiment 2: This embodiment differs from Embodiment 1 in that: the Bacillus thuringiensis HLJ-66 carries insecticidal crystal protein genes including cry1Ac, cry2Ab and cry7,8, and exotoxin cyt gene. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0036] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the active Bacillus thuringiensis HLJ-66 against Bt diamondback moth is a strain to Lepidoptera pests, anti-Btk Lepidoptera pests , Anti-Cry1Ac insecticidal crystal protein Lepidoptera pests and Coleoptera pests have high virulence Bacillus thuringiensis. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to activated bacillus thuringiensis HLJ-66 with Bt diamond back moth resistance and application thereof. The strain disclosed by the invention is bacillus thuringiensis HLJ-66, is called HLJ-66 for short and has the preservation number of CGMCCNo.8445, the preservation data of Nov. 8th in 2013 and the preservation address of No. 3, courtyard No.1, Beichen West Road, Chaoyang District of Beijing. The strain has toxicity for anti-Cry1Ac insecticidal crystal protein and Btk diamond back moth by toxicity biological assay screening. The strain is utilized to produce a high-efficiency broad-spectrum pesticide for preventing and treating the lepidopterous important pest of diamond back moth; and meanwhile, the strain has a good effect on resistance pests, is beneficial for preventing and treating resistance diamond back moth in the field, improves application value, is harmless for people, livestock and other animals, has no pollution to the environment and has excellent ecological benefits and excellent application and popularization prospect.

Description

technical field [0001] The invention relates to a Bacillus thuringiensis (Bacillus thuringiensis) HLJ-66 strain active against Btk diamondback moth and its application in the preparation of insecticides. Background technique [0002] Bacillus thuringiensis (Bt) is an aerobic Gram-positive bacterium that can produce spores in soil. In 1901, Ishiwate et al. first isolated a bacterium capable of producing crystal protein in the spore formation stage from diseased silkworm (Bombyx morl) larvae. In 1915, Berliner et al. also successfully isolated from Mediterranean mealworm (Epetia kuhniella). Out of this bacterium, at the same time named it Bacillus thuringiensis-Bt.thuringiensis. Bacillus thuringiensis can produce an insecticidal crystal toxin protein (Insecticidal Crystal Proteins, delta-endotoxin) during the formation of its spores, which is effective against Lepidoptera (Lepidoptera) insects, Homoptera (Homoptera) Insects, Coleoptera (Coleoptera) insects, Diptera (Diptera)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21A01N63/00A01P7/04C12R1/07
Inventor 杨峰山朱勋刘春光张友军李柱刚杨晶谷有芳王秀君
Owner 顶秀作物科技有限公司
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