Radix angelicae pubescentis coumarin active monomer and preparation method and application thereof

Abstract

A preparation method of a radix angelicae pubescentis coumarin active monomer is characterized by comprising the following process steps: taking radix angelicae pubescentis medicine powder, using 95% ethanol for reflux extraction for three times, recovering 95% of ethanol by reduced pressure, extracting a concentrated extraction solution successively with petroleum ether and ethyl acetate to obtain extract solutions, merging the extract solutions, recovering solvents by reduced pressure to obtain extractum with a relative density of 1.0-1.2 at the temperature of 60 DEG C; performing silica gel column chromatography separation of the extractum, using petroleum ether and ethyl acetate mixed solutions with the ratios of from 100:0 to 5:1 for gradient elution, and selecting a colorless crystalline obtained by 10:1 gradient separation, namely a compound cnidium lactone crystalline. The preparation method is simple in operation and mature in technology, the obtained cnidium lactone is large in amount, high in purity and low in cost, so that the cnidium lactone is easy to promote, is accepted by the masses of alzheimer's patients, and has a broad clinical application prospect and obvious social and economic values.

Description

technical field [0001] The invention belongs to a method for preparing a traditional Chinese medicine, in particular to a method for preparing an active monomer of a traditional Chinese medicine, which can be used for treating Alzheimer's disease. Background technique: [0002] Alzheimer's disease (AD), also known as senile dementia, is a common neurodegenerative disease among middle-aged and elderly people. It is mainly manifested as progressive memory loss and cognitive dysfunction. One of the subjects [1-2] . Its pathogenesis is not very clear, and it is generally believed that the deposition of β-amyloid (β-Amyloid, Aβ) is the main reason for the formation and development of lesions. A large number of activated microglial cells gather around AD brain lesions, which produce a large number of inflammatory mediators and cause inflammatory reactions. Excessive oxidative stress produces a large number of free radicals, which affects the metabolism and function of cells, lea...

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Problems solved by technology

There are few reports on the extraction and purification of lovage coumarin. Caiqian et al. [8] The total coumarin extract of lovage purified by supercritical extraction process, the coumarin content is slightly more than 50%, the operation process is complicated, the equipment is expensive, and it is difficult to popularize and apply; Chinese patent 200710024862.8 "a preparation method of lovage extract" , using reflux extraction and organic solvent extraction method to prepare the lovage extract, although the method is simple, but the content of coumarin in the extract obtained by repeating this method is not more than 30%, and the composition is unclear, and it is difficult to exclude the residue of organic solvent. Poor results in in vitro cell experiments

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