Culturing method for ammonia oxidizing bacteria

A technique of ammonia oxidizing bacteria and a cultivation method, which is applied in the field of ammonia oxidizing bacteria cultivation, can solve the problems of slow relative growth rate of bacteria, slow start-up of the reactor, easy contamination of miscellaneous bacteria, etc., and achieve stable and controllable agitation speed, economical and efficient Preparation, the effect of not easily contaminating miscellaneous bacteria

Active Publication Date: 2014-04-09
TIANJIN CARING TECH DEV
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Problems solved by technology

These methods often have many disadvantages, such as time-consuming, easy contamination of bacteria, slow start-up of the reactor, unstable conditions, and slow relative growth rate of the flora.

Method used

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  • Culturing method for ammonia oxidizing bacteria
  • Culturing method for ammonia oxidizing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Use a fermenter with a volume of 30L, add 19L of nitrifying bacteria culture medium, and 1L of seed solution. The control parameters are: the fermenter automatically adds 10% sodium bicarbonate solution to control the pH=7.5, the temperature=25°C, and the ventilation volume is 1.5M 3 / h, the tank pressure is 0.05MPa, and the speed is 180r / min. The fermentation culture was carried out for 5 days, the fermentation liquid was collected, and the number of ammonia-oxidizing bacteria in the fermentation liquid was detected by plate counting method to be 2.54×10 9 CFU / ml.

Embodiment 2

[0023] Use a fermenter with a volume of 30L, add 19L of nitrifying bacteria culture medium, and 1L of seed solution. The control parameters are: the fermenter automatically adds 10% sodium bicarbonate solution to control the pH=8.0, the temperature=30°C, and the ventilation volume is 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation culture was carried out for 7 days, the fermentation liquid was collected, and the number of ammonia-oxidizing bacteria in the fermentation liquid was detected by plate counting method to be 1.36×10 10 CFU / ml.

Embodiment 3

[0025] Use a fermenter with a volume of 200L, add 120L of nitrifying bacteria culture medium, and 6L of seed solution. The control parameters are: pH=8.0, temperature=30°C, air flow is 2.0M 3 / h, the tank pressure is 0.1MPa, and the speed is 200r / min. The fermentation culture was carried out for 7 days, the fermentation liquid was collected, and the number of ammonia-oxidizing bacteria in the fermentation liquid was detected by plate counting method to be 1.09×10 10 CFU / ml.

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Abstract

The invention provides a culturing method for ammonia oxidizing bacteria. The culturing method comprises the following steps: inoculating ammonia oxidizing bacteria liquid into a fermentation tank; carrying out fermentation culture for 5-7 days; collecting fermentation liquid, so as to obtain the ammonia oxidizing bacteria, wherein the inoculation amount is 5%; the culturing conditions refer to that a pH (Potential of Hydrogen) value is controlled to be 7.5-8.0 by adding a base liquid, a temperature is 25-30 DEG C, a ventilation amount is 1.5-2.0M<3>/h, a tank pressure is 0.05-0.1MPa, and a rotation speed is 180-200r/min; a culture medium is a nitrobacteria culture medium. The culturing method uses a fermentation tank yeast propagation method, so that infectious microbes are difficult to pollute; various parameters, such as the temperature, the pH value, dissolved oxygen and a stirring rotation speed and the like, can be stably controlled; compared with the traditional opened type yeast propagation method, the culturing method for the ammonia oxidizing bacteria can be used for more economically and effectively preparing an ammonia oxidizing bacteria agent, so that the agent can be rapidly and effectively propagated; the culturing method for the ammonia oxidizing bacteria is suitable for large-scale industrial production and has a wide application prospect in a sewage treatment field.

Description

technical field [0001] The invention relates to a method for cultivating bacteria, in particular to a method for cultivating ammonia-oxidizing bacteria. Background technique [0002] Ammonia nitrogen is one of the most important pollutants in water bodies: in an alkaline environment, ammonia nitrogen will cause toxicity to aquatic organisms; the oxidation of ammonia nitrogen to nitrate nitrogen and nitrite will cause water blooms; Drinking water may pose a health hazard to humans. With the development of industrial and agricultural production, the pollution of ammonia nitrogen is increasing day by day, which has become one of the urgent problems to be solved in the field of sewage treatment, and it is also one of the key environmental indicators monitored by the state. [0003] The traditional physical and chemical removal methods of ammonia nitrogen include air stripping method, selective ion exchange method based on resin or zeolite, chemical precipitation method, chemica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 薛二军李玉庆张建邓继鹏宋存江李强
Owner TIANJIN CARING TECH DEV
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