Culturing method for ammonia oxidizing bacteria

Abstract

The invention provides a culturing method for ammonia oxidizing bacteria. The culturing method comprises the following steps: inoculating ammonia oxidizing bacteria liquid into a fermentation tank; carrying out fermentation culture for 5-7 days; collecting fermentation liquid, so as to obtain the ammonia oxidizing bacteria, wherein the inoculation amount is 5%; the culturing conditions refer to that a pH (Potential of Hydrogen) value is controlled to be 7.5-8.0 by adding a base liquid, a temperature is 25-30 DEG C, a ventilation amount is 1.5-2.0M<3> / h, a tank pressure is 0.05-0.1MPa, and a rotation speed is 180-200r / min; a culture medium is a nitrobacteria culture medium. The culturing method uses a fermentation tank yeast propagation method, so that infectious microbes are difficult to pollute; various parameters, such as the temperature, the pH value, dissolved oxygen and a stirring rotation speed and the like, can be stably controlled; compared with the traditional opened type yeast propagation method, the culturing method for the ammonia oxidizing bacteria can be used for more economically and effectively preparing an ammonia oxidizing bacteria agent, so that the agent can be rapidly and effectively propagated; the culturing method for the ammonia oxidizing bacteria is suitable for large-scale industrial production and has a wide application prospect in a sewage treatment field.

Description

technical field [0001] The invention relates to a method for cultivating bacteria, in particular to a method for cultivating ammonia-oxidizing bacteria. Background technique [0002] Ammonia nitrogen is one of the most important pollutants in water bodies: in an alkaline environment, ammonia nitrogen will cause toxicity to aquatic organisms; the oxidation of ammonia nitrogen to nitrate nitrogen and nitrite will cause water blooms; Drinking water may pose a health hazard to humans. With the development of industrial and agricultural production, the pollution of ammonia nitrogen is increasing day by day, which has become one of the urgent problems to be solved in the field of sewage treatment, and it is also one of the key environmental indicators monitored by the state. [0003] The traditional physical and chemical removal methods of ammonia nitrogen include air stripping method, selective ion exchange method based on resin or zeolite, chemical precipitation method, chemica...

AI Technical Summary

Problems solved by technology

These methods often have many disadvantages, such as time-consuming, easy contamination of bacteria, slow start-up of the reactor, unstable conditions, and slow relative growth rate of the flora.

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