Method for extracting Enteromorpha pigment

A technology of pigments and enteromorpha, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve problems such as the influence of marine benthic ecosystems, changes in the physical and chemical properties of sediments, and the death of aquatic organisms, and achieve low production costs , easy operation, and the effect of reducing waste

Active Publication Date: 2015-05-27
上海松皓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enteromorpha itself is non-toxic, but the green tide formed may cause outbreaks of pests. Algae deposition on the seabed will cause hypoxia in the water body, lead to the death of aquatic organisms, and change the physical and chemical properties of the sediment, which will have a serious impact on the marine benthic ecosystem.

Method used

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  • Method for extracting Enteromorpha pigment
  • Method for extracting Enteromorpha pigment
  • Method for extracting Enteromorpha pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Harvest Enteromorpha, discard impurities, and wash with fresh water until the conductivity of the rinse solution does not exceed 0.02ms. (2) Remove moisture, heat and dry at 60°C. (3) Use a masher dry method to mash the dried Enteromorpha until the size of the fragments is 3mm. (4) Soak overnight in alcohol with a volume concentration of 75%, filter the extract with a 600-mesh sieve, take the filtrate, and rotatively evaporate the filtrate at 50°C to recover the alcohol. (5) Extract the pigment from the remaining solution with ethyl acetate, and take the upper layer solution to obtain the pigment solution.

[0023] (6) Suspend the silica gel resin with petroleum ether and install the chromatographic column. The pigment solution extracted with ethyl acetate was blotted dry with silica gel, and placed on a silica gel chromatographic column for loading.

[0024] (7) The pigment components were collected by eluting with petroleum ether as a detergent, spin-dried and ...

Embodiment 2

[0028] (1) Harvest Enteromorpha, discard impurities, and wash with fresh water until the conductivity of the rinse solution does not exceed 0.02ms. (2) Remove moisture, heat and dry at 60°C. (3) Use a masher dry method to mash the dried Enteromorpha until the size of the fragments is 3mm. (4) Soak overnight in alcohol with a volume concentration of 75%, filter the extract with a 600-mesh sieve, take the filtrate, and rotatively evaporate the filtrate at 45°C to recover the alcohol. (5) Extract the pigment from the remaining solution with ethyl acetate, and take the upper layer solution to obtain the pigment solution.

[0029] (6) Suspend the silica gel resin with petroleum ether and install the chromatographic column. The pigment solution extracted with ethyl acetate was blotted dry with silica gel, and placed on a silica gel chromatographic column for loading.

[0030] (7) First use petroleum ether as a detergent to elute until colorless, and then use petroleum ether / ethyl...

Embodiment 3

[0034] In Example 2, petroleum ether and petroleum ether / ethyl acetate mixed solvent with a volume ratio of 4:1 were used as detergents for sequential elution, and then washed with petroleum ether / ethyl acetate mixed solvent with a volume ratio of 7:3. The pigment components were collected, spin-dried and concentrated. Take samples for thin layer chromatography, dissolve them with extractant (acetone / petroleum ether=3 / 2 volume ratio), develop with developer (petroleum ether / acetone=3 / 1 volume ratio), and measure the Rf value. Sampling and scanning the absorption spectrum, using a mixed solvent of petroleum ether / ethyl acetate = 3 / 1 as a solvent and a blank control, the scanning range is 300-700nm, and the interval is 1nm. The preliminary identification of the results is as follows:

[0035] pigment

[0036] Ester a)

[0037] The identification result was chlorophyll pigment (chlorophyllin a). Use this pigment to dye white cloth, sieve silk and waterproof ...

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Abstract

The invention discloses a method for extracting an Enteromorpha pigment, which comprises the following steps: washing Enteromorpha, drying, pulverizing, and extracting by immersing in alcohol; filtering the extracting solution to obtain a filtrate, recovering the alcohol, and extracting with ethyl acetate to obtain a pigment solution; and passing the pigment solution through a silica gel resin chromatographic column, eluting by using petroleum ether or a petroleum ether-ethyl acetate mixed solution or ethyl acetate as an eluting agent, collecting the pigment component, and concentrating to remove the solvent. According to the method, the reagents used in the pigment crude extracting solution working process are water and alcohol, and the alcohol is recycled, so no environmental pollution is generated; the organic solvents used in the pigment purification process can also be recycled; and the extracted pigment has the advantages of stable properties and low production cost, is simple to operate, and has wide prospects when being developed as a food additive or staining agent.

Description

technical field [0001] The invention belongs to the field of aquatic product processing, especially algae processing, and specifically relates to the preparation of Enteromorpha pigment. Background technique [0002] Enteromorpha belongs to Chlorophyta, Chlorophyceae, Ulva Order, Ulvaceae, and is a large marine green algae. Enteromorpha has a strong ability to resist stress and grow. The green tide that has broken out in China's Yellow Sea for 7 consecutive years is the dominant species of Enteromorpha. Enteromorpha itself is non-toxic, but the green tide formed may cause outbreaks of pests. The algae deposited on the seabed will cause hypoxia in the water body, lead to the death of aquatic organisms, and change the physical and chemical properties of the sediment, which will have a serious impact on the marine benthic ecosystem. But the biomass resources contained in Enteromorpha can be exploited and utilized. Enteromorpha can be processed into moss strips for consumption...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C09B61/00C07C403/24C07D487/22
Inventor蔡春尔杨亚云何培民贾睿胡燕杨欣欣吴维宁耿中雷张林慧韦章良
Owner上海松皓生物科技有限公司