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Method for identifying commercially available orange juice beverage based on three-dimensional fluorescence spectrum and robust mahalanobis distance

A three-dimensional fluorescence and Mahalanobis distance technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of high detection cost, adulteration of fruit juice and juice drinks, poor reproducibility of results, etc.

Inactive Publication Date: 2014-04-30
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

At present, due to the imperfection of relevant standards and testing methods, adulteration of fruit juices and juice drinks has occurred from time to time. Some commercially available 100% orange juices are actually diluted with concentrated orange juice and water, and then added preservatives, thickeners, artificial flavors and other food additives. The "orange juice drink" produced, or even the "three fine water" prepared with citric acid, sweetener, orange flavor, thickener, synthetic coloring, etc., will cause damage to the interests and health of consumers
Due to the uneven quality of commercially available orange juice drinks, it is difficult for consumers to identify them through simple sensory judgments, and the components of orange juice itself are relatively complex mixtures, which are difficult to directly measure by chemical methods
At present, for the detection and identification of orange juice drinks, the national standard GB / T 16771 is usually implemented, which includes potassium, total phosphorus, amino acid nitrogen, L-proline, total D-isocitric acid and total flavones as six components The characteristic component index of orange juice, but this method has the disadvantages of cumbersome operation, time-consuming detection, high detection cost, poor reproducibility of results, etc. Therefore, it is very necessary to find a fast, convenient, stable and reliable orange juice beverage identification technology

Method used

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  • Method for identifying commercially available orange juice beverage based on three-dimensional fluorescence spectrum and robust mahalanobis distance
  • Method for identifying commercially available orange juice beverage based on three-dimensional fluorescence spectrum and robust mahalanobis distance
  • Method for identifying commercially available orange juice beverage based on three-dimensional fluorescence spectrum and robust mahalanobis distance

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Embodiment Construction

[0028] Below in conjunction with accompanying drawing and according to the operation order of flowchart, the present invention will be further described:

[0029] a. The samples are freshly squeezed orange juice, commercially available 100% orange juice, and orange juice drinks. After centrifugation, use a fluorescence spectrometer to measure the three-dimensional fluorescence spectrum of all samples. The excitation wavelength range is set to 300nm-600nm, and the step size is set to 5nm. The emission wavelength range was set to 300nm-700nm, and the step size was set to 1nm. In order to eliminate the influence of Rayleigh scattering, the emission wavelength scanning position was delayed by 15nm relative to the excitation wavelength. All samples were measured under the same parameter settings and experimental conditions. figure 2 , image 3 , Figure 4 The three-dimensional fluorescence spectra of freshly squeezed orange juice, commercially available 100% orange juice, and ora...

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Abstract

The present invention discloses a method for identifying a commercially available orange juice beverage based on a three-dimensional fluorescence spectrum and a robust mahalanobis distance. The method comprises: adopting fluorescence characteristic difference between orange juice components and food additives to establish three-dimensional fluorescence standard spectrum libraries of the freshly squeezed orange juice, the 100% qualification rate orange juice and the orange juice beverage, extracting the spectrum gravity center through a gravity center method to form the 100% orange juice standard feature database, measuring the three-dimensional fluorescence spectrum of a sample requiring identification and extracting the gravity center feature data, and calculating the robust mahalanobis distance to discriminate the sample requiring identification, wherein the discrimination threshold is 2.72. According to the method, the defects of complex process, detection time consuming, high detection cost and the like of the traditional chemical detection method are overcome, the rapid screening and identification on the sample requiring identification can be achieved, the advantages of rapidness, safety, reliability simple operation, low cost and the like are provided, the standardization on the fruit juice beverage industry and protection of consumer interests can be easily achieved, and food safety supervision on the fruit juice beverage can be promoted.

Description

technical field [0001] The invention provides a method for identifying commercially available orange juice drinks based on three-dimensional fluorescence spectrum and robust Mahalanobis distance, belonging to the field of detection and identification of fruit juice drinks. Background technique [0002] Orange juice drink is a kind of fruit juice drink that is popular among consumers. According to the juice content, orange juice drinks on the market can be roughly divided into two categories: 100% orange juice and orange juice drinks. The national standard GB / T 21731 is implemented. At present, due to the imperfection of relevant standards and testing methods, adulteration of fruit juices and juice drinks has occurred from time to time. Some commercially available 100% orange juices are actually diluted with concentrated orange juice and water, and then added preservatives, thickeners, artificial flavors and other food additives. The "orange juice drink" produced, or even the...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 陈国庆吴亚敏朱拓胡扬俊朱纯朱焯炜
Owner JIANGNAN UNIV
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