Method for determining content of multi-index components in purification process of Chinese buckeye seed extractive

A multi-indicator and content technology, applied in the field of multi-indicator component content detection, can solve problems such as rapid judgment that has not been seen, and achieve the effect of eliminating baseline drift and improving model performance.

Inactive Publication Date: 2014-05-21
SHANDONG LUYE PHARMA CO LTD
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Problems solved by technology

The moving block standard deviation method (MBSD method) is a commonly used endpoint judgment method. It has been reported in the literature that the MBSD method can be used to quickly judge the endpoint of traditional Chinese medicine extraction, but it has not been used in the rapid judgment of the endpoint of the macroporous resin elution process.

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  • Method for determining content of multi-index components in purification process of Chinese buckeye seed extractive
  • Method for determining content of multi-index components in purification process of Chinese buckeye seed extractive

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Embodiment Construction

[0024] The method for rapidly detecting the effective components in the purification process of the sodium aescin macroporous resin by near-infrared spectroscopy, the detection steps are as follows:

[0025] (1) Collection of calibration set samples during loading and elution of macroporous resin:

[0026] Collect 80 samples from different batches of macroporous resin during loading and elution

[0027] (2) Determination of the reference value of the multi-index component content of the calibration set sample

[0028] A. The content of total sodium aescinate and sodium aescinin A, B, C, D in the determination sample by HPLC method:

[0029] Chromatographic conditions: the chromatographic column is Phanomenex, C 18 (100mm×4.6mm, 2.6μm); mobile phase: A is 0.5% phosphoric acid solution, B is acetonitrile, carry out gradient elution according to Table 1; detection wavelength: 220nm; column temperature: 30 ℃; flow rate: 1ml / min.

[0030] Standard curve drawing: Accurately weigh...

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Abstract

The invention discloses a method for rapidly determining the content of multi-index components in a sample feeding and eluting process of a Chinese buckeye seed extractive, namely sodium aescinate, in macro-porous resin and rapidly judging an eluting terminal point. The method comprises the following steps: (1) scanning near infrared spectrums and collecting a macro-porous effluent liquid sample; (2) determining the content of the multi-index components in a sample by using a reference method; (3) establishing a near-infrared multi-element correction model in a macro-porous purification process; (4) carrying out model evaluation; and (5) rapidly judging the eluting terminal point. The method provided by the invention has the advantages of rapidness, no loss, capability of simultaneously detecting a plurality of components and the like, can be used for an online analysis of a traditional Chinese medicine production process, and provides a novel thought for production and quality control of traditional Chinese medicines.

Description

technical field [0001] The invention belongs to a quality detection method in the production process of traditional Chinese medicines, and relates to a detection method for rapidly determining the content of multi-index components in the macroporous resin purification process of the extract of Sala chinensis by near-infrared spectroscopy. Background technique [0002] In recent years, safety incidents of traditional Chinese medicine injections have occurred frequently, and the number of adverse reaction reports has also increased. Unstable working conditions and differences in human operation during the production of traditional Chinese medicine injections often lead to unstable quality of traditional Chinese medicine injections, and product safety cannot be guaranteed. The vital interests of patients are of great significance. [0003] Sodium aescinate for injection can be used for the treatment of swelling caused by cerebral edema trauma and surgery, and also for venous r...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
Inventor 薛云丽刘雪松范艳丽丁海婴王龙虎陈勇吴永江
Owner SHANDONG LUYE PHARMA CO LTD
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