Post-column derivatization type high-performance liquid chromatography

Abstract

The invention relates to a post-column derivatization type high-performance liquid chromatography with the high efficiency and the low cost and belongs to the technical field of chromatographic analysis. Particularly, an old or wasted liquid chromatogram pump is used as a post-column derivatization external peristaltic pump; a wasted gas chromatography or thermostat is linked with a liquid chromatograph as a post-column derivatization reaction box, so that a derivatizing agent smoothly enters a reaction system so as to ensure reaction at a suitable reaction temperature; a post filled with powdery steel powder or a powdery sand core replaces a post-column derivatization pipeline; the length of the post-column derivatization pipeline is shortened, and the surface area of the post-column derivatization reaction is enlarged, so that the reaction is sufficient; with the adoption of the derivatization chromatographic process, a post-column derivatization experiment can be smoothly carried out in a lab in the absence of the post-column derivatization system; meanwhile, the use ratio of old instruments in the lab can be improved; moreover, the post-column derivatization experiment can be carried out by adopting the process, so that the excellent derivatization effect, simple method, high efficiency and low cost can be realized.

Description

technical field

[0001] The invention relates to a post-column derivatization chromatographic method, in particular to an efficient and cheap post-column derivatization high-performance liquid chromatography method, which belongs to the technical field of chromatographic analysis. Background technique

[0002] In recent years, people have continuously improved the methods for identifying and detecting the content of substance components, and liquid chromatography (LC) is often used to separate

[0003] It is an important means of detection and identification of various substances. However, when some substances that exist in nature, such as sugars and amino acids, are detected by conventional optical detection methods, the sensitivity is often low and the selectivity is not good. In order to overcome the shortcomings of these methods and achieve high-sensitivity detection and trace analysis, indirect detection methods need to be used. Before using high-performance liquid chr...

Examples

Embodiment 1

[0020] Connect the high-performance liquid chromatograph with the peristaltic pump of the discarded liquid chromatograph, and connect it with the gas chromatograph so that the hardware conditions for post-column derivatization are equipped.

[0021] The stainless steel column filled with 10-50 μm powdered steel powder (the specific particle inner diameter is optimized and screened according to the water and substance) is used as a post-column derivation column to connect with the separation column of the high-performance liquid chromatograph, and with the waste liquid chromatograph connected with the peristaltic pump, so that the sample is preliminarily separated by the separation column and then enters the post-column derivatization column. Derivative heating is balanced to achieve the purpose of post-column derivatization.

Embodiment 2

[0023] Connect the high-performance liquid chromatograph with the peristaltic pump of the discarded liquid chromatograph, and connect it with the thermostat box, so that the hardware conditions for post-column derivatization are equipped.

[0024] The stainless steel column filled with 10-50 μm powdery sand core (the specific particle inner diameter is optimized and screened according to the water and substance) is used as a post-column derivation column to connect with the separation column of the high-performance liquid chromatography, and with the waste liquid chromatography connected with the peristaltic pump, so that the sample is preliminarily separated by the separation column and then enters the post-column derivatization column. Derivative heating is balanced to achieve the purpose of post-column derivatization.

technical field

[0001] The invention relates to a post-column derivatization chromatographic method, in particular to an efficient and cheap post-column derivatization high-performance liquid chromatography method, which belongs to the technical field of chromatographic analysis. Background technique

[0002] In recent years, people have continuously improved the methods for identifying and detecting the content of substance components, and liquid chromatography (LC) is often used to separate

[0003] It is an important means of detection and identification of various substances. However, when some substances that exist in nature, such as sugars and amino acids, are detected by conventional optical detection methods, the sensitivity is often low and the selectivity is not good. In order to overcome the shortcomings of these methods and achieve high-sensitivity detection and trace analysis, indirect detection methods need to be used. Before using high-performance liquid chr...

Examples

Embodiment 1

[0020] Connect the high-performance liquid chromatograph with the peristaltic pump of the discarded liquid chromatograph, and connect it with the gas chromatograph so that the hardware conditions for post-column derivatization are equipped.

[0021] The stainless steel column filled with 10-50 μm powdered steel powder (the specific particle inner diameter is optimized and screened according to the water and substance) is used as a post-column derivation column to connect with the separation column of the high-performance liquid chromatograph, and with the waste liquid chromatograph connected with the peristaltic pump, so that the sample is preliminarily separated by the separation column and then enters the post-column derivatization column. Derivative heating is balanced to achieve the purpose of post-column derivatization.

Embodiment 2

[0023] Connect the high-performance liquid chromatograph with the peristaltic pump of the discarded liquid chromatograph, and connect it with the thermostat box, so that the hardware conditions for post-column derivatization are equipped.

[0024] The stainless steel column filled with 10-50 μm powdery sand core (the specific particle inner diameter is optimized and screened according to the water and substance) is used as a post-column derivation column to connect with the separation column of the high-performance liquid chromatography, and with the waste liquid chromatography connected with the peristaltic pump, so that the sample is preliminarily separated by the separation column and then enters the post-column derivatization column. Derivative heating is balanced to achieve the purpose of post-column derivatization.

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