Trace gadolinium-doped manganese oxide nanoparticles for treating brain glioma

A manganese oxide and nanoparticle technology, applied in non-active ingredient medical preparations, emulsion delivery, pharmaceutical formulations, etc., can solve problems such as nervous system toxicity, and achieve the effect of prolonging imaging time and clearing tumor boundaries

Inactive Publication Date: 2014-06-04
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although manganese is a trace element needed in the body, excessive manganese can also cause toxicity to the nervous system

Method used

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  • Trace gadolinium-doped manganese oxide nanoparticles for treating brain glioma
  • Trace gadolinium-doped manganese oxide nanoparticles for treating brain glioma
  • Trace gadolinium-doped manganese oxide nanoparticles for treating brain glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Gd x mn (1-x) Preparation of O-OA

[0050] 1) Preparation of gadolinium oleate: 20mmol GdCl 3 , 60mmol sodium oleate, 30mL distilled water, 40mL ethanol, and 70mL n-hexane were added to the three-necked flask, where GdCl 3 The molar ratio with sodium oleate is 1:3, react at 70°C for 4 hours. Cool to room temperature, let stand to separate the layers, and wash the organic layer three times with distilled water. After drying, gadolinium oleate can be obtained.

[0051] 2) Preparation of manganese oleate: 20mmol MnCl 2 , 40mmol sodium oleate, 30mL distilled water, 40mL ethanol, and 70mL n-hexane were added to the three-necked flask at the same time, where MnCl 2 The molar ratio with sodium oleate is 1:2. The reaction was carried out at 70° C. for 4 hours. Cool to room temperature, let stand for liquid separation, and wash the organic phase three times with distilled water. Dry to get manganese oleate.

[0052] 3) Gd x mn (1-x) Preparation of O-OA: Di...

Embodiment 2

[0053] Example 2: Gd x mn (1-x) Preparation of O–TETT(x=0.01)

[0054] Preparation of Trace Gadolinium Doped Manganese Oxide Nanoparticles Gd in Water Dispersion by Ligand Exchange Method x mn (1-x) O–TETT(x=0.01): Add 100mg Gd x mn (1-x) Dissolve O-OA in 60 mL of anhydrous toluene, add 60 μL of acetic acid, sonicate for 15 minutes, add 0.6 mL of silane carboxylic acid (TETT), and react at 70 ° C for 48 hours. The resulting Gd x mn (1-x) O-TETT (x=0.01) was washed with toluene and anhydrous methanol three times respectively, dialyzed for 24 hours, and freeze-dried.

[0055] The product of the present invention Gd x mn (1-x) O-TETT (x=0.01) transmission electron microscope, particle size distribution and Fourier transform infrared characterization see figure 1 , 2 , 3.

Embodiment 3

[0056] Example 3: Gd x mn (1-x) Determination of Relaxation Rate of O-TETT(x=0.01)

[0057] Weigh a certain amount of Gd x mn (1-x) O-TETT (x=0.01), dispersed in 1% agarose gel, respectively prepared into solutions with concentrations of 0.5, 0.25, 0.125, 0.0625, and 0.03125 millimoles (Mn) / L. Use 7T nuclear magnetic resonance instrument, select RARE-T 1 +T 2 -map sequence, the parameters are set as follows: TR / TE=3000 / 45ms(T 2 ), TR / TE=500 / 9ms(T 1 ), FOV=4.0×4.0cm 2 And slice thickness=1mm, T 1 weighted imaging scan, the resulting transverse relaxation time (T 1 ) is linearly fitted to the concentration, and the slope obtained is the relaxation rate. Its fitting curve see Figure 4 .

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Abstract

The invention discloses trace gadolinium-doped manganese oxide nanoparticles. Gadolinium-doped manganese oxide nanoparticles are taken as crystal kernels, and the surfaces of the crystal kernels are combined with a silylating reagent; the molar ratio of gadolinium to manganese is 0.01-0.1. The invention further discloses a method for preparing the gadolinium-doped manganese oxide nanoparticles. The gadolinium-doped manganese oxide nanoparticles can be taken as a contrast agent in magnetic resonance imaging, and can be applied to medicament carriers, cell tracing medicaments and gene transfection.

Description

technical field [0001] The invention relates to a manganese oxide nanoparticle doped with a trace amount of gadolinium, and more particularly relates to a trace gadolinium-doped manganese oxide nanoparticle for brain glioma. [0002] The present invention also relates to a preparation method of the above-mentioned trace amount of gadolinium-doped manganese oxide nanoparticles. [0003] The invention also relates to the use of trace gadolinium-doped manganese oxide nanoparticles as a contrast agent in magnetic resonance imaging (MRI), as well as in drug carriers, cell tracer drugs and gene transfection. Background technique [0004] The use of contrast agents in magnetic resonance imaging (MRI) enhances the contrast between pathological and normal tissues. Gadolinium chelates currently in clinical use as T 1 Contrast agents are limited to imaging brain tumors without a blood-brain barrier and the potential for nephrogenic systemic fibrosis (NSF). Functionalized superparama...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K47/04
Inventor 叶玲邵晨李帅顾微宫宁强邓云龙肖宁
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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