System and method for constructing injected three-dimensional cell microenvironment based on microfrozen gel

A technology of micro-environment and micro-ice gel, which is applied in medical science, prosthesis, etc., can solve the problems that the two-dimensional micro-environment cannot simulate and reproduce the three-dimensional micro-environment well, reduce the survival rate of cells, and restrict the treatment effect of stem cells, etc.

Active Publication Date: 2014-06-25
BEIJING CYTONICHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

There are four reasons why direct injection causes a large number of transplanted stem cells to die and lose after entering the body: (1) During the injection and transplantation of stem cells, due to the influence of mechanical shear force in the syringe, some cells rupture, and the cells enter the apoptotic stage, reducing cell viability
(2) The free stem cells in vitro enter the damaged tissue area in the body. During this process, the microenvironment of the cells changes, and the microenvironment of the lesion area is not conducive to cell survival and growth, and the stem cells cannot adapt to the microenvironment of the lesion. thereby reducing the viability of the cells
(3) Stem cells are directly injected into the body. Due to the difference between the treatment site and the injection site, some injection sites may be due to mechanical movement or pressure difference between the inside and outside of the tissue site (such as prevertebral disc herniation disease model, cartilage tissue injury model, cardiopulmonary repair transplantation, etc.) and other models), the cells injected into the target tissue will overflow from the needle hole as the syringe is pulled out, resulting in a large number of cell loss. Oriented dissociation, unable to actively aggregate, resulting in the loss of a large number of transplanted cells, unable to perform fixed-point cell therapy
Therefore, this simplified two-dimensional microenvironment cannot simulate and reproduce the three-dimensional microenvironment in the body well.
However, depending on the stem cells cultured in the traditional two-dimensional environment, during the stem cell transplantation treatment, due to the drastic changes in the living environment in vivo and in vitro, the survival rate and function of stem cells are reduced, and the repair and regeneration functions are reduced, which restricts the use of stem cell therapy. The effect of clinical application

Method used

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  • System and method for constructing injected three-dimensional cell microenvironment based on microfrozen gel
  • System and method for constructing injected three-dimensional cell microenvironment based on microfrozen gel
  • System and method for constructing injected three-dimensional cell microenvironment based on microfrozen gel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Embodiment 1, Microcryogel three-dimensional microenvironment carrier and preparation method thereof

[0101] 1. Preparation of the mold for preparing microcryogel three-dimensional microenvironment carrier (referred to as microcryogel mold)

[0102] Micro ice glue mold structure: It is a flat plate with concave holes; the concave holes are arranged in an array, 45x14 array distribution, each concave hole is cylindrical, and the cylindrical cross section is a circle with a diameter of 400um, each circular The distance between the center of the circle is 1.5mm, and the height of the concave hole is 300μm. The plate material is PMMA.

[0103] Using laser cutting method, the specific process is as follows:

[0104] The present embodiment selects the polymethyl methacrylate (PMMA) plate making mold that thickness is about 300 μ m;

[0105] Use AutoCAD software to draw, draw a rectangle with a length of 75cm and a width of 25cm, and draw a circle with a diameter of 400um ...

Embodiment 2

[0125] Embodiment 2, the performance detection of three-dimensional microenvironment carrier of microcryogel

[0126] The microcryogel three-dimensional microenvironment carriers used in the following experiments all refer to the microcryogel three-dimensional microenvironment carriers obtained in Example 1.

[0127] 1. The three-dimensional microenvironment carrier of microcryogel remains intact during the injection process, indicating that its mechanical properties are good

[0128] METHODS: The micro-cryogel injection experiment was carried out, and the mechanical properties of the micro-cryogel were studied by observing the integrity of the three-dimensional micro-environment carrier of the micro-cryogel, the thrust during injection, and whether there was clogging during the injection process. The specific implementation method is as follows:

[0129] Preparation of suspended microcryogel injection: resuspend the microcryogel three-dimensional microenvironment carrier wit...

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PUM

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Abstract

The invention discloses a system and a method for constructing an injected three-dimensional cell microenvironment based on microfrozen gel. A microfrozen gel three-dimensional microenvironment vector is made of the frozen gel and in micro scale size. Experiments demonstrate that the prepared microfrozen gel three-dimensional microenvironment vector has the following characteristics that mechanical properties are good; whole morphology can be kept after being injected; the vector can load cells effectively; compared with conventional large-scale frozen gel, the microfrozen gel three-dimensional microenvironment vector can absorb the cells uniformly and has good material transmission performance; the microfrozen gel three-dimensional microenvironment vector has a protection effect for loaded cells in an injection process; and the microfrozen gel loaded with the cells can be injected into an animal body at specific sites and keep original functions and activities in in-vivo cells. The microfrozen gel three-dimensional microenvironment vector plays an important role in the field of cell injection therapy, and has wide application prospects in animal model establishment and the drug screening field.

Description

technical field [0001] The invention provides a system and method for constructing an injection-type three-dimensional cell microenvironment based on microcryogel. Background technique [0002] Regenerative medicine represented by cell therapy has gradually become an important direction in the development of clinical medicine, bringing new hope for complex and serious diseases that are difficult to be effective in drug treatment. Cell therapy is a treatment method that uses the patient's own (or allogeneic) adult cells or stem cells to repair tissues and organs. At present, there have been a large number of reports on the use of cells to treat chronic diseases such as cardiovascular diseases, diabetes, Parkinson's disease, corneal lesions, cartilage tissue damage, and tumors, and preliminary results have been achieved in clinical applications. In particular, many breakthroughs have been made in the research and application of stem cell therapy. Stem cells are a type of plu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/16A61L27/20A61L27/24A61L27/22A61L27/38A61L27/56
Inventor 杜亚楠刘伟李雅倩
Owner BEIJING CYTONICHE BIOTECH CO LTD
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