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Formula of culturing agent for producing DNA from Crypthecodinium cohnii

A culture agent, the technology of Cryptodinia, applied in the biological field, can solve problems such as the culture agent for extracting DHA, and achieve the effects of high DHA yield and large biomass

Inactive Publication Date: 2014-06-25
QINGDAO ZHONGREN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

And there is not yet a kind of formula that effectively extracts the culture agent of DHA from dinoflagellates

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0013] The formulation of the culture agent for producing DHA from Cryptidinium algae of the present embodiment, its components are proportioned by mass: 8 parts of peptone, 2 parts of sodium nitrate, 20 parts of glucose, 5 parts of sodium acetate, 6 parts of sodium chloride, sulfuric acid Magnesium 1.6 parts, VH 0.006 parts, VB (12) 0.001 parts, water 1000 parts.

[0014] Put 200mL culture agent configured by the present invention in the 500mL Erlenmeyer flask, under all black conditions, the initial temperature is 30 DEG C, after cultivating for 32 hours, gradually cool down to 20 DEG C, continue to cultivate for 32 hours, harvest, the biomass is 10.78g / L, the yield of DHA was 3.21 g / L, and the yield and substrate conversion coefficient were 0.489 g / L·d.

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PUM

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Abstract

The invention discloses a formula of a culturing agent for producing DNA from Crypthecodinium cohnii. The formula comprises, by mass, 7.2-8.8 parts of peptone, 1.8-2.2 parts of sodium nitrate, 16-22 parts of glucose, 4.5-5.5 parts of sodium acetate, 5.4-6.6 parts of sodium chloride, 1.44-1.76 parts of magnesium sulfate, 0.0054-0.0066 parts of VH, 0.0009-0.0011 parts of VB(12), and 900-1100 parts of water. Crypthecodinium cohnii is cultured according to the formula in order to realize high DHA output and large biomass.

Description

[0001] technical field [0002] The invention belongs to the field of biotechnology, and in particular relates to the formula of a culture agent for producing DHA from Cryptidinosa. [0003] Background technique [0004] Crypthecodinium cohnii is an important class of phytoplankton in the ocean. They belong to eukaryotes, but they retain some prokaryotic characteristics and have some special characteristics. They are intermediate transitions between prokaryotes and eukaryotes type. It is rich in DHA, its content is as high as 50% of fatty acids, and the composition of fatty acids is simple, which is very conducive to the extraction of DHA, so it has great industrial advantages. And its fatty acid hardly contains EPA, so it is very suitable for adding to infant food. At present, there is no effective formulation of a culture agent for extracting DHA from dinoflagellates. [0005] Contents of the invention [0006] The object of the present invention is to provide a for...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/12C12R1/89
Inventor 张述智夏伟朱绍辉张浩王晓丽徐权汗李之详许团辉
Owner QINGDAO ZHONGREN PHARMA
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