Novel explant used in plant tissue culture and sterilizing method for novel explant

The technology of plant tissue culture and sterilization method is applied in the field of selection of explants in the process of plant tissue culture.

Inactive Publication Date: 2014-07-09
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Because the sterilizing solvent of the prior art has great damage to the young shoots of the plants, the young shoots cannot survive

Method used

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Embodiment Construction

[0016] The present invention takes the young shoots of the plants during the growing season, immerses them in the garlic aqueous solution to sterilize them, inoculates them on a suitable medium for cultivation, observes, counts and analyzes the aseptic conditions, growth and development of the young shoots after a period of time . The young shoots of plants are less capable of resisting external environmental disturbances, and the sterilizing solutions of the prior art do more damage to them, thus limiting the possibility of the young shoots being used as explants. For this reason, the present invention provides a kind of novel sterilizing solution---the aqueous solution that utilizes garlic preparation. Specifically, peeled garlic is mashed into a puree, and a solution of 1 to 9:1 is prepared with sterile single distilled water in a weight-to-volume ratio. The sterilizing solution is mild and suitable for sterilizing young shoots of plants as explants. Here, the young shoot...

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Abstract

The invention discloses a novel explant used in plant tissue culture. A tender shoot in the vegetation season is taken as an explant. The tender shoot is a segment of new shoot or branch of 5-10 centimeters in length below the stem top of a plant in the vegetation season. The invention further discloses a sterilizing method of the tender shoot explant. The sterilizing method comprises the following steps: preparing a sterilizing aqueous solution by using garlic; and soaking the tender shoot into the solution for sterilizing. The tender shoot in the vegetation season is taken as the explant for tissue culture, so that the application range of the plant tissue culture explant is expanded; the inoculation time of the explant under a natural condition is prolonged; compared with conventional leaf bud and stem tissue culture, in the invention, the combination of the tender shoot in the vegetation season and the garlic solution has the advantages that the tissue culture time is shortened, the tissue culture cost is reduced, and the operation is relatively easy.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, more specifically, relates to the selection of explants in the process of plant tissue culture. Background technique [0002] Organs or tissue sections that can be induced to produce clonal lines are called explants. The explants used in current scientific research and production practice include leaf buds, stem segments attached to leaf buds, root segments, leaves, protoplasts, embryos, endosperms, anthers, ovaries, and the like. [0003] The selection of plant explants needs to be carried out according to the selection of the growing season and the appropriate local location of the plant. In the prior art, most of those skilled in the art select dormant shoots as explants instead of new shoots in the growing season. Because the sterilizing solvent of the prior art damages the tender shoots of the plants relatively, the tender shoots cannot survive. Therefore those skilled in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 侯义龙于亚军张立娟倪天泽
Owner DALIAN UNIV
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