Method for measuring N-acetylglucosamine by utilizing ion exchange chromatography

A technology of ion exchange chromatography and acetylamino, which is applied in the field of quantitative analysis for the determination of N-acetylglucosamine and N-acetylglucosamine by ion exchange chromatography, and can solve the problems of difficult accurate quantification, large error, and large influence, etc. Achieve the effect of reducing analysis time, high accuracy and improving accuracy

Inactive Publication Date: 2014-07-09
NANJING FORESTRY UNIV
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Problems solved by technology

[0007] The above chemical methods all use color reaction to determine the content of N-acetylglucosamine in the sample. The color reaction is greatly affected by the color of the sample itself, so it is difficult to accurately quantify and the error is large.

Method used

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  • Method for measuring N-acetylglucosamine by utilizing ion exchange chromatography
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  • Method for measuring N-acetylglucosamine by utilizing ion exchange chromatography

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Embodiment 1

[0024] 1) High performance liquid ion exchange chromatography system and chromatographic conditions

[0025] High performance liquid phase ion exchange chromatography system: American Dionex ICS-5000 ion chromatography system, equipped with double pump (DP) built-in vacuum degassing module, electrochemical detector (ED) and automatic sampler (Dionex AS-DV), chromatography system The operating software used Chromeleon 6.80 chromatographic workstation; chromatographic conditions: chromatographic column: Dionex AminoPac PA10 column (2×250mm); guard column is Dionex AminoPac PA10 (2×50mm); column temperature: 30°C; injection volume: 25.0μL , the flow rate is 0.25mL / min.

[0026] Elution conditions: use 10mmol / L sodium hydroxide as the eluent for isocratic elution.

[0027] Signal detection: The detection mode of the electrochemical detector is gold working electrode and pH-Ag / AgCl compound reference electrode, and the content of N-acetylglucosamine is determined by integral and p...

Embodiment 2

[0035] Example 2 Quantitative analysis of N-acetylglucosamine components in actual samples

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Abstract

The invention discloses a method for measuring N-acetylglucosamine by utilizing ion exchange chromatography. The method comprises the following steps: on an ion exchange chromatography, detecting by adopting a DionexAminPacPA10 chromatographic column with the temperature of 30 DEG C, taking 10mmol/L of sodium hydroxide as a mobile phase and using an electrical conductivity detector; drawing a concentration-peak area standard curve of the N-acetylglucosamine by adopting an external standard method; calculating to obtain the content of the N-acetylglucosamine. A method for accurately and quantitatively measuring the N-acetylglucosamine through high performance ion exchange chromatography is established at first, the DionexAminPacPA10 chromatographic column is adopted, and the separation degree and detection efficiency of the N-acetylglucosamine are obviously improved through isocratic elution of the 10mmol/L of sodium hydroxide. The method is high in precision, high in reproducibility and high in accuracy and can realize direct sample detection, the sample is not needed to be subjected to other pretreatment, the analysis time is greatly reduced, the accuracy, convenience and reliability of the detection method are improved, and the method has significance for detection, evaluation, popularization and application of N-acetylglucosamine products.

Description

technical field [0001] The invention belongs to the technical field of quantitative analysis of chemical substances, and relates to a quantitative analysis method of N-acetylglucosamine, in particular to a method for determining N-acetylglucosamine by ion exchange chromatography. Background technique [0002] N-acetylglucosamine is an important hydrolysis product of chitin and chitosan, and N-acetylglucosamine has been widely used in the field of medicine as a drug for treating osteoarthritis and rheumatoid arthritis. In addition, N-acetylglucosamine is also widely used as a low-calorie sweetener in the field of food additives. [0003] At present, the following methods are mainly used for the quantitative determination of N-acetylglucosamine: [0004] (1) 3,5-dinitrosalicylic acid (DNS method). This method has a better color development effect for N-acetylglucosamine, but the sensitivity of this method is low, and the detection range is small, between 200-1000 ug / L. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/60G01N30/64G01N30/02
Inventor 勇强杨磊李鑫陈牧连之娜徐勇余世袁
Owner NANJING FORESTRY UNIV
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