A kind of preparation method of verbena flat spike artificial seed
A technology of verbenia flat tachycarpus and artificial seeds, which is applied in the field of making artificial seeds of verbena tachycarpus, can solve the problems of low germination rate of artificial seeds and cannot meet the needs of ecological environment construction, and achieve fast propagation speed, easy acquisition, The effect of high survival rate
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Embodiment 1
[0032] In this embodiment, the solvent of the artificial endosperm in the semi-gel state is MS medium solution, the mass volume ratio of the activated carbon is 0.5%, the mass volume ratio of sodium alginate is 2%, 3%, 4%, A completely randomized experimental design was adopted, with a total of 3 treatments, and 20 artificial seeds were produced in each treatment group, and the 20 artificial seeds prepared in each treatment group were placed in a petri dish covered with wet filter paper, placed in a place where the temperature could be controlled manually. Cultivate in a light culture room with environmental conditions such as light intensity. The light intensity is 2000lx, 12h / d, and the temperature is 25°C. After 15 days from the date of sowing, the artificial seed characteristics and germination rate are counted, as shown in Table 1:
[0033] Table 1
[0034]
[0035] Note: The statistical method of the germination rate: the artificial seeds were placed in a petri dish c...
Embodiment 2
[0039] In this embodiment, the solvent of the artificial endosperm in the semi-gel state is MS medium solution, the mass volume ratio of the activated carbon is 0.5%, the mass volume ratio of sodium alginate is 3%, and the content of NAA is 0, 0.1mg / L, 0.2mg / L, 0.3mg / L, hormone GA 3 The content of cytokinin 6-BA was 0, 0.01mg / L, 0.05mg / L, 0.1mg / L, and the content of cytokinin 6-BA was 0, 1mg / L, 2mg / L, 3mg / L. A completely randomized experimental design was adopted. A total of 16 treatments, 20 artificial seeds were made for each treatment group, and the 20 artificial seeds made by each treatment group were placed in a petri dish covered with wet filter paper, placed in a room where the temperature, light intensity and other environmental conditions could be controlled manually. Cultivate in the light culture room, light intensity is 2000lx, 12h / d, temperature 25 ℃, since the date of sowing 15d after statistics artificial seed characteristics and germination rate, as shown in Ta...
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