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Fast erythrocyte removal method applicable to whole blood detection

A technology of red blood cells and red blood cells, which is applied in the field of medicine and health, and can solve problems such as the inability to achieve full blood testing

Inactive Publication Date: 2014-10-22
叶森
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Another object of the present invention is to provide a sample processing kit, which can s

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  • Fast erythrocyte removal method applicable to whole blood detection
  • Fast erythrocyte removal method applicable to whole blood detection

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[0023] Example

[0024] 1. Experiment purpose:

[0025] Verify the application of the method for quickly removing red blood cells described in the present invention to assist the chemiluminescence in vitro diagnostic kit in detecting whole blood samples.

[0026] 2. Experimental equipment and reagents:

[0027] Chemiluminescence instrument (Shenzhen new industry maglumi), constant temperature water bath (HH-60, Changzhou Guohua Electric Co., Ltd.), micropipette (Thermo company), magnetic separation rack (Tianjin Best Chromatography Technology Development Center). PCT calibrator (Audit company), PBS-T buffer (KH 2 PO 4 3.35g / L, Na 2 HPO 4 -12H 2 O 17.9g / L, KCl 0.2g / L, NaCl8.77g / L, Tween-20 0.5g / L, pH7.2), PCT calibrator diluent is PBS-T buffer containing 20% ​​bovine serum, PCT The preparation of the detection kit (Shenzhen New Industry) and the whole blood processing kit is the same as the preparation method described in the instructions.

[0028] 3. Experimental steps:

[0029] 3.1 C...

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Abstract

The invention relates to the field of medicine and health and provides a method for simple, rapid and effective separation of erythrocytes. According to the method, immunological agglutination technology and magnetic separation technology are combined, an antigen-antibody compound is formed by an ovine antibody against human erythrocytes and an erythrocyte, then the antigen-antibody compound and a magnetic microsphere coated by a mouse monoclonal antibody against ovine Fc-terminal undergo immunoadsorption so as to induce a more intense agglutination reaction, and magnetic separation is carried out so as to achieve the purpose of removal of erythrocytes. The method realizes simple and rapid removal of erythrocytes in samples and can be applied on a fully-automatic or semi-automatic chemiluminescence apparatus so as to realize whole blood detection of a common kit.

Description

technical field [0001] The invention relates to the field of medicine and health, and provides a simple, fast and effective method for separating red blood cells. Background technique [0002] The current clinical serum separation method is to separate the red blood cells, white blood cells, and platelets from the serum by centrifuging the blood collection tubes for 30 minutes after blood collection. Due to the certain centrifugal force generated during the centrifugation process, this centrifugal force will lead to the accumulation of red blood cells, resulting in the rupture of cells and hemolysis. Centrifugation is a complicated process, especially when the sample volume is large, and it is difficult for even skilled operators to ensure the uniformity of each sample. Related literature reports that hemolysis caused by improper centrifugation accounted for 41.2% of hemolysis. [0003] Red blood cells will interfere with the performance of immunomagnetic beads in conventi...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/54333G01N2800/324
Inventor 叶森
Owner 叶森
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