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Preparation method and use of environmental estrogen label-free immunosensor

An environmental estrogen and immunosensor technology is applied in the field of preparation of environmental estrogen unlabeled immunosensors, and can solve the problems of poor specificity, complicated operation, expensive instruments and the like, and achieve the effects of convenient operation, simple preparation, and highly selective detection.

Inactive Publication Date: 2014-11-05
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the detection methods have expensive instruments and complicated operations, and the inspectors must undergo strict training before testing
Some simple methods, such as electrochemical analysis, are simple, sensitive and fast, but have poor specificity.

Method used

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  • Preparation method and use of environmental estrogen label-free immunosensor
  • Preparation method and use of environmental estrogen label-free immunosensor
  • Preparation method and use of environmental estrogen label-free immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 CoFe 2 o 4 Preparation of / rGo

[0033] 8 mg graphene oxide was ultrasonically dispersed into 25 ml absolute ethanol, and 0.5 ml 0.2 mol / mL Fe(NO 3 ) 3 aqueous solution, 0.25 ml of 0.2 mol / L Co(NO 3 ) 2 aqueous solution and 2.0 ml ammonia water, stirred at 80°C for 10h, then transferred to an autoclave, and reacted at 180°C for 4h; after cooling to room temperature, centrifuged and dried in a vacuum oven at 50°C to obtain CoFe 2 o 4 / rGo.

Embodiment 2

[0034] Example 2 CoFe 2 o 4 Preparation of / rGo

[0035] Ultrasonic dispersion of 10 mg graphene oxide into 30 ml of absolute ethanol was followed by adding 1 ml of 0.2 mol / mL Fe(NO 3 ) 3 Aqueous solution, 0.5 ml of 0.2 M Co(NO 3 ) 2 aqueous solution and 2.50 ml of ammonia water, stirred at 80°C for 12h, then transferred to an autoclave, and reacted at 180°C for 5h; cooled to room temperature, centrifuged and dried in a vacuum oven at 50°C to obtain CoFe 2 o 4 / rGo.

Embodiment 3

[0036] Example 3 CoFe 2 o 4 Preparation of / rGo

[0037] 12 mg of graphene oxide was ultrasonically dispersed into 35 ml of absolute ethanol, and 1.5 ml of 0.2 mol / mL Fe(NO 3 ) 3 aqueous solution, 0.75 ml of 0.2 mol / L Co(NO 3 ) 2 aqueous solution and 3.0 ml ammonia water, stirred at 80°C for 14h, then transferred to an autoclave, and reacted at 180°C for 6h; cooled to room temperature, centrifuged, and dried in a vacuum oven at 50°C to obtain CoFe 2 o 4 / rGo.

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Abstract

The invention discloses a preparation method of an environmental estrogen label-free immunosensor and a use of the environmental estrogen label-free immunosensor in environmental estrogen detection, and belongs to the technical field of novel functional nano-materials and environment detection. The preparation method utilizes the two novel functional nano-materials to modify working electrodes, utilizes synergetic catalysis effects and antibody sorption effects of the two novel functional nano-materials, and prepares the environmental estrogen label-free immunosensor which has high sensitivity, good specificity and fast detection rate, is prepared by simple processes and is used for environmental estrogen detection.

Description

technical field [0001] The invention relates to a preparation method and application of an environmental estrogen label-free immunosensor. Specifically, it is a label-free electrochemical immunosensor for detecting environmental estrogen constructed by a new nanocomposite material with a synergistic catalytic effect, which belongs to the technical field of new nanometer functional materials and environmental detection. Background technique [0002] Environmental estrogen is a kind of chemical substance that exists in the environment. It has the activity similar to that of estrogen in organisms. After entering the human body, it has the effect of simulating estrogen. It is a class of environmental toxins that seriously endanger human health. Common sources of environmental estrogen include pesticides (such as organochlorine pesticides), second-hand smoke, waste gas (such as toxic gases produced by burning plastic waste), food additives, etc. [0003] The detection methods f...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N27/327
CPCG01N27/416G01N33/5438G01N33/74
Inventor 张勇郭占魁魏琴吴丹李燕
Owner UNIV OF JINAN
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