Temporary planting and seedling hardening method of dendrobium tissue culture seedlings

Abstract

The invention discloses a temporary planting and seedling hardening method of dendrobium tissue culture seedlings, and relates to the technical field of dendrobium cultivation. The method includes the following steps that (1) by means of moderate microclimate conditions under protected cultivation frame overhead planting bed, ground under the bed serves as a dendrobium temporary planting seedbed; (2) culture medium main raw materials are fermented; (3) culture media are prepared and put into a seedling hole plate; (4) the dendrobium tissue culture seedlings of the right age are selected for indoor seedling hardening; (5) the tissue culture seedlings are placed on the temporary planting seedbed after temporary planting on the seedling hole plate; (6) management after temporary planting is carried out. After the method is adopted, the temporary planting survival rate of the dendrobium tissue culture seedlings is high, the cultured hole plate seedlings are strong and uniform, seedling survival is easy after planting, growth is quick, the method is suitable for seedlings for dendrobium protected cultivation and bionic cultivation large-scale production, and the utilization rate of equipment can be improved.

Description

technical field [0001] The invention belongs to the technical field of dendrobium cultivation, and in particular relates to a method for artificially planting and strengthening seedlings of dendrobium tissue culture seedlings. Background technique [0002] Dendrobium (Dendrobium), also known as Dendrobium orchid, is a perennial epiphytic herbaceous plant of the genus Dendrobium in the family Orchidaceaceae. It is an important medicinal and ornamental plant. , the origin is mainly distributed in tropical and subtropical Asia, Australia and Pacific islands, there are more than 1,500 species in the world, and about 76 species in China, most of which are distributed in Southwest, South China, Taiwan and other places, of which nearly 40 species have medicinal properties value. The types of Dendrobium in my country only account for about 5% of the world, but its medicinal development and utilization are at the forefront of the world. Dendrobium is a traditional Chinese medicinal ...

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Problems solved by technology

The seeds of Dendrobium candidum are extremely small, with incomplete embryonic development and lack of endosperm tissue. Under natural conditions, they need to coexist with some fungi (blue bacteria) to germinate, and the germination rate is extremely low. For example, the seed germination rate of Dendrobium candidum is less than 5%.

Its defect is that the first three items all use peat as the main raw material of the matrix, while peat resources in my country are relatively small and are non-renewable natural resources. Although the research of Liu Shihui et al. did not use peat as the main raw material of the matrix, the The nutrients in the plant are very little, and the requirements for fertilization management after artificial planting are high, requiring "thin fertilizer and frequent application". Not only does it need to spray liquid fertilizer multiple times, but also apply granular slow-release fertilizer to help Dendrobium candidum grow vigorously; at the same time, These 4 studies all need special facilities for artificial planting and hardening. Because the adaptability of tissue culture seedlings is poor and the requirements for environmental conditions are high, the requirements for facilities and the level of cultivation management are also high. The facilities for planting and hardening seedlings are relatively simple or the management is extensive, and the survival rate of fake planting of tissue culture seedlings is still relatively low

However, the current method of protected cultivation of Dendrobium in my country generally adopts elevated cultivation beds for cultivation. Many protected cultivation flowers, especially some high-end orchid varieties such as Phalaenopsis, are also cultivated with elevated cultivation beds. The ground and space are generally idle and useless, which is a big waste of cultivation facilities

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