Tissue Culture and Rapid Propagation Method of Fenza 1 Fenjiao
A technology for tissue culture, fast propagation and powdered bananas, applied in the field of plant tissue culture, can solve the problems of low efficiency, slow reproduction, and low survival of rooted seedling transplantation.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0046] Implementation location: Female parent garden of Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences, time: October-November 2012.
[0047](1) Screening of mother plants: select new banana plants with green, strong pseudostems, a height of 3.2-3.5m, larger ears, less conjoined fruit, normal fruit shape, and good quality, and plant them in greenhouses or In the isolated plantation, it is used as the female parent garden for budding.
[0048] (2) Treatment of explant sucking buds: when the sucking buds grow to 30-50cm, spray the mixed liquid of 50% carbendazim 1000 times and 150ppm agricultural streptomycin on the sucking buds. Specifically, 1,000 kg of mixed medicinal solution contains 1 kg of 50% carbendazim, 150 grams of agricultural streptomycin, and 0.5 kg of mixed medicinal solution per bud; 1 week later, the suction buds are taken as explants for inoculation.
[0049] (3) Differentiation culture: Glass bottles or polypropylene film bags are...
Embodiment 2
[0057] Implementation location: The female parent garden of the Institute of Fruit Trees, Guangdong Academy of Agricultural Sciences, time: October 2013.
[0058] (1) Screening of the mother plant: Select the newly planted banana with a green, strong pseudostem and a height of 3.4m and the perennial banana with a height of 4.1m. The ears are larger, the fruit fingers are less conjoined, the fruit shape is normal, and the quality is good. , as the female parent tree for budding.
[0059] (2) Treatment of sucking buds of explants: when the sucking buds grow to 30-50cm, spray the mixed liquid of 50% carbendazim 1000 times solution plus 150ppm agricultural streptomycin on the sucking buds, and pour the mixed medicine into each bud 0.5 kg of liquid; 1 week later, the suction buds were taken as explants for inoculation.
[0060] (3) Differentiation culture: Polypropylene film bags are used as packaging containers for differentiation culture, and the temperature is controlled at 29°...
Embodiment 3
[0066] Implementation location: The mother garden of the Institute of Fruit Trees, Guangdong Academy of Agricultural Sciences, time: October 2014.
[0067] (1) Screening of mother plants: select perennial Banana plants with green, strong pseudostems, a height of 4.0m, larger ears, less conjoined fruit, and normal fruit shape, as the female parent tree, for bud picking .
[0068] (2) Treatment of sucking buds of explants: When the sucking buds grow to 40cm, spray the mixed liquid of 50% carbendazim 1000 times solution plus 150ppm agricultural streptomycin on the sucking buds, and pour 0.5 kg; 1 week later, the sucking buds were taken as explants for inoculation.
[0069] (3) Differentiation culture: Glass bottles are used as packaging containers for differentiation culture, and the temperature is controlled at 29°C to 33°C. Differentiate and cultivate for 17 days to subculture. When subculture transfers differentiated buds, cut off the upper part of the sheath leaf that cuts ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com