Vacuum storage method and application of soybean pollen

A preservation method and pollen technology, applied in the field of plant physiology, can solve the problems of affecting pollination efficiency, high preservation cost, shortening pollination time, etc., and achieve the effect of avoiding unfavorable pollination environmental conditions, high preservation cost and high equipment requirements

Active Publication Date: 2014-11-19
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defect of conventional method is: 1) soybean pollen vigor can only be maintained in 2-4 hours under natural conditions, so the pollination time of soybean hybridization is limited to 2-4 hours in the morning of pollination, if run into rainy, dewy weather, Pollination time will also be shortened, affecting pollination efficiency
Vacuum cryopreservation of pollen is one of the methods for off-site pollination of plants, non-pollination during flowering and preservation of germplasm resources. However, due to the high vacuum requirements and the low temperature is usually below -70°C, the preservation cost is expensive. Usually, vacuum cryopreservation of pollen is only used for Rare plants, not popular in hybrid seed production of conventional crops

Method used

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  • Vacuum storage method and application of soybean pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the method for pollen preservation of the present invention and the detection of pollen vitality after preservation

[0032] 1. Storage method

[0033] In Wuhan, spring soybean varieties Tianlong 1 and Yudou 22 were selected, sown on April 3, and entered the full flowering period on May 20. At 7-8 o'clock in the morning, select flower buds that are about to open that day on the male parent plant, put them in a 9*2.5cm petri dish, and use absorbent paper to absorb the moisture on the flower buds for the flower buds back, 50 flower buds per dish.

[0034] Put the petri dish with flower buds into a 15*15cm vacuum bag, use a Prestige household vacuum packaging machine to pump out the air in the bag, seal it, and store the vacuum bag in a 4°C refrigerator.

[0035] 2. Pollen viability detection

[0036] Take out pollen from vacuum bag after different time, place on glass slide, drip a drop of culture solution (200mg / L boric acid solution+2% sucrose solution),...

example 2

[0039] Example 2, pollen preservation method and hybridization efficiency of the present invention:

[0040] In Wuhan area, the spring soybeans Tianlong 1 and Yudou 1 were selected, sown in early April, and entered the full flowering period in late May, with Yudou 1 as the male parent and Tianlong 1 as the female parent. Preserve the pollen according to the method described in Example 1.

[0041] Before pollination, perform the following operations: take the flower buds out of the vacuum bag, place them under a 60W incandescent lamp at 20cm for 20 minutes, take out 3 flower buds every 5 minutes after 20 minutes, peel off the sepals and corolla, and check whether the pollen has been released from the anthers If it has been released, it can be used for pollination; if it has not been released, it will continue to be irradiated with incandescent lamps. The female parent was pollinated at different time intervals after pollen collection, and 50 flowers were pollinated at each int...

example 3

[0045] Example 3, the detection of different genotype hybridization efficiency

[0046]In Wuhan, spring soybeans Zhongdou No. 40 and Brazil No. 10 were selected, sown in early April, and bloomed in late May. Brazil No. 10 was used as the male parent, and Zhongdou 40 was used as the female parent. Preserve the pollen according to the method described in Example 1.

[0047] Before pollination, perform the following operations: take the flower buds out of the vacuum bag, place them under a 60W incandescent lamp at 20cm for 20 minutes, take out 3 flower buds every 5 minutes after 20 minutes, peel off the sepals and corolla, and check whether the pollen has been released from the anthers If it has been released, it can be used for pollination; if it has not been released, it will continue to be irradiated with incandescent lamps. After pollen collection, the female parent was pollinated in the morning and afternoon of the same day, and 50 flowers were pollinated at each time peri...

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Abstract

The invention discloses a vacuum storage method and application of soybean pollen. The method comprises the following specific operation steps of putting a flower bud in a vacuum bag, completely pumping the air in the bag, sealing the vacuum bag and storing the vacuum bag at 4 DEG C. Experiments prove that when the soybean pollen is stored by using the vacuum storage method, the vigor preservation time of the pollen is prolonged from 2-4 hours to 48 hours, so that the effective pollination time is prolonged, the degree of freedom of the pollination time is increased, the environment unfavorable to the development of fertilized eggs is avoided, the labor intensity is reduced, the work efficiency is improved and a foundation is laid for the hybrid seed production and the genetic research.

Description

technical field [0001] The invention relates to the field of plant physiology, in particular to a vacuum preservation method and application of soybean pollen. Background technique [0002] Hybrid breeding is one of the main approaches to crop improvement. Soybean is a self-pollinating plant, and hybrid seed production is accomplished through artificial emasculation. The method usually adopted is: at 16-19 o'clock in the afternoon the day before pollination or at 8-11 o'clock in the morning on the day of pollination, select flower buds that are about to bloom the next day on the female plant, remove sepals, corollas and stamens with tweezers, At this time, the flowers that are about to bloom on the same day are taken from the male plant, the stamens are taken out, and the pollen is dotted on the emasculated pistil. The defect of conventional method is: 1) soybean pollen vigor can only be maintained in 2-4 hours under natural conditions, so the pollination time of soybean h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00A01H1/02
Inventor 单志慧杨中路陈海峰沙爱华邱德珍张婵娟陈李淼袁松丽周蓉陈水莲周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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