Method for Determining Virulence of Predator Mite

A measurement method, a technology of predatory mite, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of high escape rate of predatory mite, strong self-activity, and difficult to guarantee the accuracy of results, so as to avoid suffocation and death, and is convenient to manufacture , the result is accurate and reliable

Active Publication Date: 2016-01-20
CITRUS RES INST SOUTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to their small size, strong mobility and lack of bristles on the back, predatory mites cannot learn from traditional methods for determining the toxicity of spider mites such as the leaf residue method.
At present, the method for evaluating the toxicity of predatory mite is mainly the leaf residue method, which is adopted by IOBC (The International Organization for Biological Control, International Organization for Biological Control), and is also listed as an official evaluation method by EPPO (European and Mediterranean Plant Protection Organization, European and Mediterranean Plant Protection Organization). , but this method has a high escape rate of predatory mites, and the accuracy of the obtained results is difficult to guarantee

Method used

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  • Method for Determining Virulence of Predator Mite
  • Method for Determining Virulence of Predator Mite
  • Method for Determining Virulence of Predator Mite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Determination of the toxicity of chlorpyrifos and lambda-cyhalothrin to Neoseius pasteurii

[0026] 1. Material

[0027] 1.1 Source of tested mite

[0028] The subject of the experiment was the new small seiusi Pasteur, collected from lemon leaves around the orchard of the Citrus Research Institute of the Chinese Academy of Agricultural Sciences.

[0029] 1.2 Chemical reagents: see Table 1

[0030] Table 1 Chemical reagents to be tested

[0031]

[0032] 2 method

[0033] 2.1 Preparation of reagents with different concentration gradients

[0034] Different concentration gradients were prepared by diluting the field concentration of predatory mites in the instructions for use of the chemical reagents. The different concentration gradients of chlorpyrifos and lambda-cyhalothrin set in this experiment are shown in Table 2. Each reagent has 8 different settings The concentration gradient.

[0035] 2.2 Operation method for virulence determination

[0036] For each pesticide, 8 t...

Embodiment 2

[0054] Example 2 Comparison of escape rate between the leaf residue method and the method of the invention

[0055] The escape rate of the traditional leaf residue method and the method of the present invention are compared. Each method was used to detect 40 new mites of Pasteurella mite, with 3 replicates for each method. After 24 hours, count the mites on the leaves and in the device of the present invention to calculate the escape rate.

[0056] As shown in Table 3, it can be seen from the results of the significance analysis that the escape rate of the new mites Pasteurii using the method of the present invention is significantly lower than that of the leaf residue method, indicating that the method of the present invention is used to detect the resistance of predator mites. More accurate and reliable.

[0057] Table 3 Comparison of escape rate between the leaf residue method and the predator mite virulence measuring device of the present invention

[0058]

[0059] Remarks: *As...

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Abstract

The invention discloses a predatory mite toxicity testing method which comprises the following steps: (1) keeping predatory mites at minus 20 DEG C for 5min so that the predatory mites are under a cold paralytic state, picking the predatory mites on a nylon screen above 300 meshes through a fine writing brush, fully soaking the nylon screen attached with the predatory mites in an agent to be tested and keeping for 5-10s; and then, lifting the nylon screen, placing on a piece of dry filter paper and absorbing residual solution; (2) opening an upper cover of a predatory mite toxicity testing device, putting the nylon screen with the predatory mites in the step 1 onto a mite supporting screen in the predatory mite toxicity testing device, covering the testing device, placing the device on a tray that contains water, breeding the tray in an artificial incubator, observing and recording death rate of the predatory mites after a period and calculating a toxicity testing result. The predatory mite toxicity testing method disclosed by the invention is simple and convenient to operate and is accurate and reliable in result.

Description

Technical field [0001] The invention relates to a technical method for monitoring the resistance of agricultural beneficial insects, in particular to a method for determining the toxicity of predatory mites. Background technique [0002] Predator mites are one of the good natural enemies of biological control. They can prey on various pests (mites) such as spider mites, greenhouse whiteflies and thrips. It has the advantages of safety and no pollution, and has been widely used in fruit trees, tea and vegetables abroad. The prevention and control of other economic crop pests is also a natural enemy product promoted by the green prevention and control of crop pests (mites) in my country. However, chemical control is still an indispensable pest control measure in production. The application of chemical pesticides inevitably leads to the contradiction between chemical control and biological control. In particular, the application of broad-spectrum insecticides such as organophosphoru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
Inventor 丛林冉春陈飞刘浩强李鸿筠田慧霞于士将杨涓丁莉莉
Owner CITRUS RES INST SOUTHWEST UNIV
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