Biological beautifying preparation containing autologous stroma cells

Abstract

The invention provides a method for preparing a biological beautifying preparation containing autologous stroma cells. The method comprises the following steps: acquiring autologous plasma from autologous peripheral blood and culturing to acquire autologous stroma cells; and adding the autologous stroma cells into the autologous plasma to prepare the biological beautifying preparation. The invention simultaneously provides the biological beautifying preparation prepared from the autologous stroma cells and the autologous plasma, which is added with growth factors and anti-oxidation factors, and is capable of removing skin wrinkles, repairing acne scarring, repairing and improving burns, trauma and hypertrophic scars, resisting senescence and the like.

Description

technical field

[0001] The present invention relates to a method for preparing an autologous biocosmetic preparation and an autologous biocosmetic formulation obtained by the method. In particular, the present invention relates to the use of autologous peripheral blood-derived serum and stromal cells, with the addition of growth factors and antioxidant molecules, and biocosmetic preparations obtained by the method. The biological cosmetic preparation of the present invention can be used for skin wrinkle removal, acne scar repair, burn, trauma and contracture scar, and anti-aging repair and improvement. Background technique

[0002] At present, there are various kinds of biological beauty products on the market, which have become popular products that are generally accepted by people. The vast majority of biological beauty products are mainly made of chemical reagents, such as latex, cream, lotion and other beauty products, which may add various nutrients, such as deep-sea c...

Examples

preparation example 1

[0047] Preparation Example 1 Preparation of human autologous plasma

[0048] Use 10ml EDTA anticoagulation blood collection tube (BD Company, USA) to extract 100ml anticoagulant meridian blood, centrifuge 10 10ml anticoagulant tubes at 1500 rpm for 5 minutes, use a 1ml pipette tip to suck and centrifuge the upper plasma and place it in a 50ml centrifuge tube , 40ml of autologous plasma was obtained and stored in the refrigerator at 4°C for later use.

preparation example 2

[0049] Preparation Example 2 Acquisition of Human Autologous Stromal Cells

[0050] Take 60ml of blood cells obtained from autologous plasma by centrifugation in Preparation Example 1, dilute with 60ml 1×PBS (pH value is 7.4), add it to the lymphatic separation solution, and conduct density gradient centrifugation at 1500rpm for 25min to obtain mononuclear cells, and use α-MEM medium. (containing 10% autologous plasma and 20ng / ml recombinant human basic fibroblast growth factor), resuspended at 3×10 6 cells / ml, added to 75cm 2 15ml in a culture flask, at 37°C, 5% CO 2 Grow in incubator for 48 hours.

[0051] After 48 hours, the culture medium was changed, the culture medium was discarded with a 10ml pipette, and 10ml of fresh α-MEM medium (containing 10% autologous plasma and 20ng / ml recombinant human basic fibroblast growth factor) was added, 75cm 2 The flask was kept at 37°C, 5% CO 2 Grow in incubator for 72 hours.

[0052] Stromal cells at 75cm after 72 hours 2 When ...

Embodiment 3

[0056] Example 3 Preparation of autologous stromal cell bio-cosmetic preparation

[0057] Take 5ml of the prepared autologous plasma, add 1ml of the prepared stromal cell fluid, and add 200ng / ml of growth factors, namely epidermal growth factor, basic fibroblast growth factor or keratin 18, and 200ng / ml of anti-inflammatory drugs. The oxidizing molecule is one of superoxide dismutase, placenta extract and ginkgo biloba extract.

technical field

[0001] The present invention relates to a method for preparing an autologous biocosmetic preparation and an autologous biocosmetic formulation obtained by the method. In particular, the present invention relates to the use of autologous peripheral blood-derived serum and stromal cells, with the addition of growth factors and antioxidant molecules, and biocosmetic preparations obtained by the method. The biological cosmetic preparation of the present invention can be used for skin wrinkle removal, acne scar repair, burn, trauma and contracture scar, and anti-aging repair and improvement. Background technique

[0002] At present, there are various kinds of biological beauty products on the market, which have become popular products that are generally accepted by people. The vast majority of biological beauty products are mainly made of chemical reagents, such as latex, cream, lotion and other beauty products, which may add various nutrients, such as deep-sea c...

Examples

preparation example 1

[0047] Preparation Example 1 Preparation of human autologous plasma

[0048] Use 10ml EDTA anticoagulation blood collection tube (BD Company, USA) to extract 100ml anticoagulant meridian blood, centrifuge 10 10ml anticoagulant tubes at 1500 rpm for 5 minutes, use a 1ml pipette tip to suck and centrifuge the upper plasma and place it in a 50ml centrifuge tube , 40ml of autologous plasma was obtained and stored in the refrigerator at 4°C for later use.

preparation example 2

[0049] Preparation Example 2 Acquisition of Human Autologous Stromal Cells

[0050] Take 60ml of blood cells obtained from autologous plasma by centrifugation in Preparation Example 1, dilute with 60ml 1×PBS (pH value is 7.4), add it to the lymphatic separation solution, and conduct density gradient centrifugation at 1500rpm for 25min to obtain mononuclear cells, and use α-MEM medium. (containing 10% autologous plasma and 20ng / ml recombinant human basic fibroblast growth factor), resuspended at 3×10 6 cells / ml, added to 75cm 2 15ml in a culture flask, at 37°C, 5% CO 2 Grow in incubator for 48 hours.

[0051] After 48 hours, the culture medium was changed, the culture medium was discarded with a 10ml pipette, and 10ml of fresh α-MEM medium (containing 10% autologous plasma and 20ng / ml recombinant human basic fibroblast growth factor) was added, 75cm 2 The flask was kept at 37°C, 5% CO 2 Grow in incubator for 72 hours.

[0052] Stromal cells at 75cm after 72 hours 2 When ...

Embodiment 3

[0056] Example 3 Preparation of autologous stromal cell bio-cosmetic preparation

[0057] Take 5ml of the prepared autologous plasma, add 1ml of the prepared stromal cell fluid, and add 200ng / ml of growth factors, namely epidermal growth factor, basic fibroblast growth factor or keratin 18, and 200ng / ml of anti-inflammatory drugs. The oxidizing molecule is one of superoxide dismutase, placenta extract and ginkgo biloba extract.

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