Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fulvic acid and antibiotic combination for the inhibition or treatment of multi-drug resistant bacteria

A technology of multi-drug-resistant bacteria and fulvic acid, which can be applied in the direction of antibacterial drugs, medical preparations containing active ingredients, and pharmaceutical formulas, and can solve problems such as harmfulness to the human body

Inactive Publication Date: 2014-12-10
NATRACINE UK
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, oxidized fulvic acids derived from coal contain high concentrations of heavy metals, including aluminum, mercury, cadmium, chromium, and lead, which are known to be harmful to humans and should be avoided in pharmaceutical preparations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fulvic acid and antibiotic combination for the inhibition or treatment of multi-drug resistant bacteria
  • Fulvic acid and antibiotic combination for the inhibition or treatment of multi-drug resistant bacteria
  • Fulvic acid and antibiotic combination for the inhibition or treatment of multi-drug resistant bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Antibacterial activity of fulvic acid against multi-drug resistant bacteria

[0034] summary

[0035] The efficacy of CHD-FA against multidrug-resistant Enterobacteriaceae in vitro was evaluated. After delivery, the active ingredients of CHD-FA were stored at room temperature and protected from light.

[0036] method

[0037] a) Strains

[0038] Antimicrobial susceptibility testing was performed on a series of multidrug-resistant Enterobacteriaceae strains. Details of the strains used are listed in Table 1.

[0039] b) Resurrection and growth of the strain

[0040] All strains were recovered from long-term storage at -80°C by subculture on CLED agar plates. Plates were then incubated in air at 35-37°C for 24 hours. Isolates were deemed suitable for use after visual inspection to ensure purity and appropriate colony characteristics.

[0041]Table 1: Enterobacter strains containing NDM-1 and KPC (Study 1)

[0042]

[0043]

[0044] c) Prepare the...

Embodiment 2

[0064] Example 2 - Efficacy of CHD-FA alone or in combination with carbapenem antibiotics in the mouse thigh model of Klebsiella pneumoniae infection

[0065] method

[0066] a) Supervision

[0067] All animal experiments were carried out under the permission of the UK Home Office and the approval of the local ethics committee. All experiments were performed by technicians who had completed parts 1, 2 and 3 of the Ministry of the Interior individual licensing course and held a current individual license.

[0068] b) mouse strain

[0069] Mice used in this study were provided by Charles River UK and are specific pathogen free. The mouse strain used was CD1, which is a well-characterized outbred strain. Mice were delivered at Euprotec's facility at 16-18 g and allowed to acclimatize for 7 days (target body weight at the start of the experiment was 22-25 g).

[0070] c) Animal feeding

[0071] Mice were housed in sterile individually ventilated cages (http: / / www.tecniplast....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Fulvic acid as the active ingredient is used in the treatment or inhibition of mutti-drug resistant bacteria, in particular NDM-1 bacteria producing carbapenemase or extended- spectrum beta-lactamase (ES8L) resistant bacteria. The multi-drug resistant bacteria may be gram negative bacteria including, but not limited to, Klebsiella pneumoniae or Escherichia coli.. The fuivic acid can be provided in combination with one or more antibiotics from the class of carbapenems or polymyxin antibiotics.

Description

Background of the invention [0001] The invention relates to the use of fulvic acid as an active ingredient for treating or inhibiting multidrug-resistant bacteria, especially carbapenemase-producing NDM-1 bacteria or extended-spectrum beta-lactamase (ESBL) drug-resistant bacteria. The present invention also relates to the use of fulvic acid as an active ingredient in combination with one or more antibiotics of the carbapenems or polymyxin antibiotics for the treatment of multidrug-resistant bacteria, especially carbapenemase-producing NDM- 1 bacteria. [0002] Fulvic acid is part of the humus formed during decay of plant and animal residues in the environment (MacCarthy et al., 1985), and it is soluble in water at all pH values. It generally has a lower molecular size and molecular weight, as well as a lower color, than humic acids, which are also found in humic substances. [0003] Typically, fulvic acids are produced from bituminous coal using a controlled wet oxidation pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/352A61K45/06A61P31/04
CPCA61K45/06A61K38/12A61K31/352A61K31/407A61K31/194A61P31/04Y02A50/30A61K2300/00
Inventor 斯蒂芬·威廉·雷弗斯彼得·沃恩
Owner NATRACINE UK
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com