Kit used for detecting double resistance of aspirin and clopidogrel drugs

A technology of clopidogrel and aspirin, which is applied in measuring devices, anti-enzyme immunoglobulins, instruments, etc., can solve the problems of platelet resistance function detection, which is difficult to be widely used clinically, and the detection method has large error and low specificity.

Active Publication Date: 2014-12-24
HANGZHOU S EVANS BIOSCI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on dual resistance to aspirin and clopidogrel is still very limited. There is a lack of detection methods for dual resistance to aspirin and clopidogrel in patients with coronary heart disease after PCI, and there is also a lack of large-scale randomized clinical studies to evaluate the effect of

Method used

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  • Kit used for detecting double resistance of aspirin and clopidogrel drugs
  • Kit used for detecting double resistance of aspirin and clopidogrel drugs
  • Kit used for detecting double resistance of aspirin and clopidogrel drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The preparation of embodiment 1 kit, component:

[0054] (1) ELISA plate coated with CK-MB antibody;

[0055] (2) Enzyme-labeled CK-MB specific antibody: labeled with horseradish peroxidase;

[0056] (3) CK-MB standard solution: 0.75μg / L, 10ml / bottle;

[0057] (4) Substrate chromogenic solution: tetramethylbenzidine; 8ml / bottle;

[0058] (5) Stop solution: 3% hydrochloric acid buffer solution; 10ml / bottle, 1 bottle;

[0059] (6) Concentrated washing liquid: deionized water; 100ml / bottle;

[0060] (7) Concentrated complex solution: phosphate buffer solution containing 1% bovine serum albumin, 10ml / bottle, 1 bottle.

[0061] Among them: the preparation of the enzyme-linked plate coated with anti-CK-MB antibody:

[0062] (1) Dilute the anti-CK-MB antibody at a concentration of 0.01 μg / ml with 0.5 mol / L carbonate buffer solution of pH 8.5 to form an antibody diluent;

[0063] (2) Add 100 μl antibody diluent to the enzyme-linked plate, incubate at 37°C for 2 hours, pou...

Embodiment 2

[0069] The preparation of embodiment 1 kit, component:

[0070] (1) ELISA plate coated with CK-MB antibody;

[0071] (2) Enzyme-labeled CK-MB specific antibody: labeled with horseradish peroxidase;

[0072] (3) CK-MB standard solution: 4.5μg / L, 10ml / bottle;

[0073] (4) Substrate chromogenic solution: tetramethylbenzidine; 8ml / bottle;

[0074] (5) Stop solution: 3% hydrochloric acid buffer solution; 10ml / bottle, 1 bottle;

[0075] (6) Concentrated washing liquid: deionized water; 100ml / bottle;

[0076] (7) Concentrated complex solution: phosphate buffer solution containing 1% bovine serum albumin, 10ml / bottle, 1 bottle.

[0077] Among them: the preparation of the enzyme-linked plate coated with anti-CK-MB antibody:

[0078] (1) Dilute the anti-CK-MB antibody at a concentration of 0.03 μg / ml with 0.5 mol / L carbonate buffer solution of pH 9.0 to form an antibody diluent;

[0079] (2) Add 100 μl of antibody diluent to the enzyme-linked plate, incubate at 37°C for 2 hours, p...

Embodiment 3

[0084] The preparation of embodiment 3 kit, component:

[0085] (1) ELISA plate coated with CK-MB antibody;

[0086] (2) Enzyme-labeled CK-MB specific antibody: labeled with horseradish peroxidase;

[0087] (3) CK-MB standard solution: 7.0μg / L, 10ml / bottle;

[0088] (4) Substrate chromogenic solution: tetramethylbenzidine; 8ml / bottle;

[0089] (5) Stop solution: 3% hydrochloric acid buffer solution; 10ml / bottle, 1 bottle;

[0090] (6) Concentrated washing liquid: deionized water; 100ml / bottle;

[0091] (7) Concentrated complex solution: phosphate buffer solution containing 1% bovine serum albumin, 10ml / bottle, 1 bottle.

[0092] Among them: the preparation of the enzyme-linked plate coated with anti-CK-MB antibody:

[0093] (1) Dilute the anti-CK-MB antibody at a concentration of 0.05 μg / ml with 0.5 mol / L carbonate buffer solution of pH 10 to form an antibody diluent;

[0094] (2) Add 100 μl antibody diluent to the enzyme-linked plate, incubate at 37°C for 2 hours, pour ...

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Abstract

The invention discloses a kit used for detecting double resistance of aspirin and clopidogrel drugs. The kit comprises (1) an assay plate coated by CK-MB (creatine kinase-MB) antibodies; (2), an enzyme labeled CK-MB specific antibody adopting horse radish peroxidase labelling; (3), a CK-MB standard solution; (4), a substrate developing solution adopting tetramethyl benzidine; (5), a stop solution adopting a hydrochloric acid buffer solution; (6), a concentration washing solution adopting deionized water; and (7), a concentration reconstitution fluid adopting a phosphate buffer solution containing 0.1%-5% of bovine serum albumin. The kit detecting sample adopts serum and can be preserved for a long time, the detecting process is simple and rapid, and the accuracy of the kit can be higher than 95%.

Description

technical field [0001] The invention relates to a detection kit, in particular to a kit for detecting dual resistance to aspirin and clopidogrel. Background technique [0002] The combined application of aspirin and clopidogrel can significantly prevent the occurrence of cardiovascular adverse events and reduce mortality in patients with coronary heart disease, especially those with coronary syndrome who have undergone percutaneous coronary intervention (PCI). In Europe and the United States, as well as domestic guidelines for the treatment of coronary heart disease, aspirin and clopidogrel both play a pivotal role. However, with the popularization and deepening of the use of aspirin and clopidogrel and other drugs, aspirin resistance is often accompanied by clopidogrel resistance, so the risk of thromboembolic events in these patients after PCI increases. Some high-risk PCI patients have dual resistance when given dual antiplatelet therapy of aspirin and clopidogrel. These...

Claims

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Application Information

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IPC IPC(8): G01N33/543C07K16/40
CPCC07K16/40G01N33/543
Inventor 吴晓星宋艳绒陈文珍杨军
Owner HANGZHOU S EVANS BIOSCI LTD
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