Composition for repairing cartilage tissue, method for producing same, and use thereof
A cartilage tissue and composition technology, applied in the field of cartilage tissue repair composition, can solve the problems of inability to provide collagen mixed composition, inability to promote cartilage regeneration, inconvenience, etc., to improve quality and reliability, relieve pain, Facilitate the evoked effect
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Embodiment 1
[0072] Application of a collagen and hyaluronic acid blend product on cartilage deficient areas in animals
[0073] Objective: To examine the applicability of an injectable collagen and hyaluronic acid mixed composition in cartilage-deficient areas of pig knees.
[0074] 1. Prepare collagen at concentrations of 5, 10, 20, 30 and 60 mg / mL and hyaluronic acid at concentrations of 5, 10 and 20 mg / mL.
[0075]Collagen concentration: As the product of the present invention is used for cartilage repair, the collagen needs to have a concentration of 5 mg / mL or more in order to maintain a state where the cartilage defect area is filled with collagen. Due to the process characteristics (sterile preparation) in the preparation of products for medical purposes (injectable form), it is difficult to prepare, practically use and apply collagen above 60 mg / mL with an ultrahigh viscosity. For the above reasons, the concentration of collagen is set in the range of 5 mg / L to 60 mg / L.
[0076]...
Embodiment 2
[0103] Experiment for examining the viscosity of a composition in which collagen and hyaluronic acid are mixed.
[0104] 1. The same method as in Example 1 was used to prepare the collagen (1, 3 and 6%) and the hyaluronic acid of 5, 10 and 20 mg / mL with a concentration of 10, 30 and 60 mg / mL in a syringe (0.5, 1 and 2%). In addition, prepare a sample in which 60 mg / mL of collagen and 5 mg / mL of hyaluronic acid are mixed in a ratio of 9:1 to 1:9 and a sample of mixing 60 mg / mL of collagen and 20 mg / mL of hyaluronic acid in a ratio of 5:5 is prepared. acid samples. At this time, the effect of the composition of collagen and hyaluronic acid mixed is equivalent to the effect due to the properties of collagen (biocompatible cartilage components and biodegradation) and due to the properties of hyaluronic acid (lubricity) Addition of the effects, thus preparing a composition suitable for cartilage restoration.
[0105] 2. Measurement of viscosity depending on the mixing ratio of c...
Embodiment 3
[0121] A test to verify the water washability of collagen and hyaluronic acid after implantation.
[0122] Purpose: To examine the resistance to interruption of the continuous adhesion (fixation) and the factors related to the filling of the injected product in the defective area.
[0123] 1. The same method as in Example 1 was used to prepare the collagen (3 and 6%) and the hyaluronic acid (1.0 and 2.0%) of 10 and 20 mg / mL in a syringe with a concentration of 30 and 60 mg / mL .
[0124] 2. After fixing the pig leg on the bracket, damage the articular cartilage by using a surgical tool, and then induce a large (about 4cm) by using a drill 2 ) and small (about 2cm 2 ) defect area.
[0125] 3. Inject and fill a filled syringe of each concentration of product into the cartilage defect area of the porcine knee.
[0126] 4. Spray water for injection down 10 cm above the injection site by using a syringe (25 mL).
[0127] 5. Visually observe the resistance of the product filli...
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