Composition for repairing cartilage tissue, method for producing same, and use thereof

A cartilage tissue and composition technology, applied in the field of cartilage tissue repair composition, can solve the problems of inability to provide collagen mixed composition, inability to promote cartilage regeneration, inconvenience, etc., to improve quality and reliability, relieve pain, Facilitate the evoked effect

Inactive Publication Date: 2014-12-31
SEWON CELLONTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] That is, there is a serious problem in the prior art that it is impossible to provide a composition mixed with biocompatible collagen for new cartilage regeneration
[0019] In addition, the prior art is inconvenient because the mixed composition cannot be applied in an injectable form, so that the cartilage tissue site with damage cannot be stabilized conveniently and efficiently, and thus cannot promote the induction of cartilage regeneration. question

Method used

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  • Composition for repairing cartilage tissue, method for producing same, and use thereof
  • Composition for repairing cartilage tissue, method for producing same, and use thereof
  • Composition for repairing cartilage tissue, method for producing same, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Application of a collagen and hyaluronic acid blend product on cartilage deficient areas in animals

[0073] Objective: To examine the applicability of an injectable collagen and hyaluronic acid mixed composition in cartilage-deficient areas of pig knees.

[0074] 1. Prepare collagen at concentrations of 5, 10, 20, 30 and 60 mg / mL and hyaluronic acid at concentrations of 5, 10 and 20 mg / mL.

[0075]Collagen concentration: As the product of the present invention is used for cartilage repair, the collagen needs to have a concentration of 5 mg / mL or more in order to maintain a state where the cartilage defect area is filled with collagen. Due to the process characteristics (sterile preparation) in the preparation of products for medical purposes (injectable form), it is difficult to prepare, practically use and apply collagen above 60 mg / mL with an ultrahigh viscosity. For the above reasons, the concentration of collagen is set in the range of 5 mg / L to 60 mg / L.

[0076]...

Embodiment 2

[0103] Experiment for examining the viscosity of a composition in which collagen and hyaluronic acid are mixed.

[0104] 1. The same method as in Example 1 was used to prepare the collagen (1, 3 and 6%) and the hyaluronic acid of 5, 10 and 20 mg / mL with a concentration of 10, 30 and 60 mg / mL in a syringe (0.5, 1 and 2%). In addition, prepare a sample in which 60 mg / mL of collagen and 5 mg / mL of hyaluronic acid are mixed in a ratio of 9:1 to 1:9 and a sample of mixing 60 mg / mL of collagen and 20 mg / mL of hyaluronic acid in a ratio of 5:5 is prepared. acid samples. At this time, the effect of the composition of collagen and hyaluronic acid mixed is equivalent to the effect due to the properties of collagen (biocompatible cartilage components and biodegradation) and due to the properties of hyaluronic acid (lubricity) Addition of the effects, thus preparing a composition suitable for cartilage restoration.

[0105] 2. Measurement of viscosity depending on the mixing ratio of c...

Embodiment 3

[0121] A test to verify the water washability of collagen and hyaluronic acid after implantation.

[0122] Purpose: To examine the resistance to interruption of the continuous adhesion (fixation) and the factors related to the filling of the injected product in the defective area.

[0123] 1. The same method as in Example 1 was used to prepare the collagen (3 and 6%) and the hyaluronic acid (1.0 and 2.0%) of 10 and 20 mg / mL in a syringe with a concentration of 30 and 60 mg / mL .

[0124] 2. After fixing the pig leg on the bracket, damage the articular cartilage by using a surgical tool, and then induce a large (about 4cm) by using a drill 2 ) and small (about 2cm 2 ) defect area.

[0125] 3. Inject and fill a filled syringe of each concentration of product into the cartilage defect area of ​​the porcine knee.

[0126] 4. Spray water for injection down 10 cm above the injection site by using a syringe (25 mL).

[0127] 5. Visually observe the resistance of the product filli...

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Abstract

The present invention relates to a composition for repairing cartilage tissue, to a method for producing same, and to the use thereof. The present invention provides a composition for repairing cartilage tissue, which is obtained by mixing collagen and hyaluronic acid using a two-way syringe or a mixer, wherein the collagen has a diluted concentration of 5-60 mg / mL excepting water or a physiological phosphate buffer solution, and the hyaluronic acid has a diluted concentration of 5-20 mg / mL excepting water or a physiological phosphate buffer solution.

Description

technical field [0001] The present invention relates to compositions for cartilage tissue repair, methods for preparing the compositions and methods for using the compositions. And more specifically, a composition based on collagen as a biomaterial is prepared in an injectable form, the composition can be implanted into a cartilage defect area, and thus, the composition can be inserted through a syringe needle without surgical incisions. direct insertion of the drug into the application site to enable the promotion of tissue repair, thereby inducing effective cartilage tissue regeneration, and thus, relatively easy and rapid induction of cartilage tissue regeneration while reducing the stress associated with surgery in animals other than humans. Restoration and regeneration in order to be able to significantly improve the quality and reliability of products, thus meeting the various expectations (needs) of consumers who use them and making a good impression on patients. Back...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/20A61F2/30
CPCA61L27/26A61L2400/06A61L2430/06A61K31/728A61P19/02C08L5/08C08L89/06A61L27/24A61L27/20A61F2/30A61K38/39A61M5/31A61M2210/02
Inventor 吕世根张晶皓柳志喆徐东三李准根禹尚勋
Owner SEWON CELLONTECH CO LTD
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