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Multilayer gradient biofilm and preparation method thereof

A biofilm and gradient technology, applied in drug delivery, pharmaceutical formulation, medical science, etc., can solve the problems of insufficient mechanical properties of natural polymers and excessive degradation speed, and achieve good cell and tissue compatibility and good biophase Capacitance, the effect of promoting bone tissue regeneration

Active Publication Date: 2019-09-03
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is: the present invention provides a multi-layer gradient biofilm and its preparation method. On the one hand, by blending natural polymers such as gelatin and collagen with other synthetic polymers and using appropriate cross-linking agents for controllable cross-linking Combined to prepare nanofibrous membranes with certain mechanical strength, in order to solve the shortcomings of insufficient mechanical properties and excessive degradation speed of a single natural polymer; And the nanofibrous membrane with calcium phosphate added and without added calcium phosphate is combined to form a multi-layer nanofibrous membrane, which not only provides better mechanical properties but also can simultaneously induce soft and hard tissue regeneration in two directions.

Method used

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  • Multilayer gradient biofilm and preparation method thereof
  • Multilayer gradient biofilm and preparation method thereof
  • Multilayer gradient biofilm and preparation method thereof

Examples

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Embodiment 1

[0057] A kind of multilayer gradient biofilm that preferred embodiment of the present invention provides, its preparation method specific steps are as follows:

[0058] Dissolve 0.06g of polycaprolactone and 0.54g of gelatin in 5mL of trifluoroethanol under stirring at 25°C, add 0.015mL of acetic acid after they are completely dissolved and stir well to obtain a spinning polycaprolactone / gelatin Solution A: Dissolve 0.30g polycaprolactone and 0.30g gelatin in 5mL trifluoroethanol under stirring at 25°C, add 0.015mL acetic acid after they are completely dissolved and stir well to obtain polycaprolactone / gelatin spinning solution B; at 25°C, 0.0325g of nano-hydroxyapatite was added to 5mL of trifluoroethanol solution, ultrasonically oscillated at 350W for 60min, and then 0.455g of polycaprolactone and 0.195g of polycaprolactone were mixed under stirring Gelatin was dissolved in trifluoroethanol solution of hydroxyapatite to prepare spinning solution C of nano-hydroxyapatite / poly...

Embodiment 2

[0061] A kind of multilayer gradient biofilm that preferred embodiment of the present invention provides, its preparation method specific steps are as follows:

[0062] Dissolve 0.18g of polycaprolactone and 0.42g of gelatin in 5mL of trifluoroethanol under stirring at 25°C, add 0.015mL of acetic acid after they are completely dissolved and stir well to obtain a spinning polycaprolactone / gelatin Solution A: Dissolve 0.30g polycaprolactone and 0.30g gelatin in 5mL trifluoroethanol under stirring at 25°C, add 0.015mL acetic acid after they are completely dissolved and stir well to obtain polycaprolactone / gelatin Spinning solution B; at 25°C, add 0.065g nano-hydroxyapatite to 5mL trifluoroethanol solution, ultrasonically shake at 350W for 60min, then mix 0.455g polycaprolactone and 0.195g polycaprolactone under stirring Gelatin was dissolved in trifluoroethanol solution of hydroxyapatite to prepare spinning solution C of nano-hydroxyapatite / polycaprolactone / gelatin. Under the co...

Embodiment 3

[0065] A kind of multilayer gradient biofilm that preferred embodiment of the present invention provides, its preparation method specific steps are as follows:

[0066] Dissolve 0.30g of polycaprolactone and 0.30g of gelatin in 5mL of trifluoroethanol under stirring at 25°C, add 0.015mL of acetic acid after they are completely dissolved and stir well to obtain the spinning polycaprolactone / gelatin Solution A: Dissolve 0.30g polycaprolactone and 0.30g gelatin in 5mL trifluoroethanol under stirring at 25°C, add 0.015mL acetic acid after they are completely dissolved and stir well to obtain polycaprolactone / gelatin spinning solution B; at 25°C, add 0.0975g nano-hydroxyapatite to 5mL trifluoroethanol solution, ultrasonically shake at 350W for 60min, then mix 0.455g polycaprolactone and 0.195g polycaprolactone under stirring Gelatin was dissolved in trifluoroethanol solution of hydroxyapatite to prepare spinning solution C of nano-hydroxyapatite / polycaprolactone / gelatin. Under the...

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Abstract

The invention discloses a multilayer gradient biofilm and a preparation method thereof. The biofilm is prepared by electrospinning and then processes such as drying, cross-linking, and sterilization.The nanofiber biofilm has a gradient change in composition and structure, and the biofilm having a gradient structure is constructed by changing the ratio of gelatin to polycaprolactone. The mechanical properties and degradation behavior can be controlled, and the upper and lower layers of the biofilm are provided with the function of synchronously guiding soft and hard tissue regeneration. The gradient biofilm has controllable mechanical properties, degradation behavior and good biocompatibility, can simultaneously induce soft and hard tissue regeneration, and has application prospects in guiding tissue regeneration.

Description

technical field [0001] The invention belongs to the technical field of nanofibrous membrane preparation, and in particular relates to a multilayer gradient biological membrane and a preparation method thereof. Background technique [0002] Simulating the structure and composition of human tissue by using appropriate materials and techniques is one of the important goals of biomimetic methods in the field of biomaterial engineering and tissue engineering. The electrospinning method can simply and effectively prepare micro-nano fiber scaffolds. This scaffold has a unique microstructure, appropriate mechanical properties and a structure similar to the natural extracellular matrix, so that it can achieve bionic structural characteristics. Therefore, electrospinning has become a One of the most promising technologies in tissue engineering applications. In addition, nanofibrous biofilms prepared by electrospinning can not only mimic the structure and environment of the extracellu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/26A61L27/46A61L27/58D04H1/413D04H1/4382D04H1/728
CPCA61L27/26A61L27/46A61L27/58A61L2400/12D04H1/413D04H1/4382D04H1/728C08L89/00C08L67/04
Inventor 李吉东任欣金蜀鄂李玉宝左奕
Owner SICHUAN UNIV
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