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A preparation method of cement-based material with deep self-healing function

A cement-based material and a self-repairing technology, which is applied in the fields of microbiology and civil engineering materials, can solve problems such as endangering structural safety, micro-crack damage, and reducing the service life of structures, achieving good repairing effect, stable properties, and good compatibility Effect

Active Publication Date: 2016-03-16
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Cement-based materials, especially concrete, due to long-term use and the influence of the surrounding complex environment, will produce local damage such as tiny cracks, which will reduce the service life of the structure in the slightest, and endanger the safety of the structure in severe cases

Method used

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  • A preparation method of cement-based material with deep self-healing function
  • A preparation method of cement-based material with deep self-healing function
  • A preparation method of cement-based material with deep self-healing function

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preparation example Construction

[0028] The invention provides a preparation method of cement-based material with deep self-repair function, the specific operation is as follows:

[0029] (1) Sieve 2000-3000 g of carriers with a particle size of 0.3-4.75 mm, wash them with tap water for 2-3 times, and dry them for later use.

[0030] (2) Prepare 2000-3000mL liquid culture medium. Taking Bacillus colloidus as an example, each liter of medium contains 8-12g of sucrose, Na 2 HPO 4 12H 2 O2~3g, MgSO 4 0.4~0.6g, CaCO 3 0.5~1.5g, KCl0.1~0.2g, (NH 4 ) 2 SO 4 0.4-0.6g, yeast extract 0.2-0.4g, and adjust the pH to 7±0.5. Inoculate carbonic anhydrase-producing microorganisms into the sterilized medium, culture at 30±0.5°C for 36-48 hours, centrifuge in a high-speed refrigerated centrifuge at a speed of 4000-6000r / min, and concentrate the bacterial solution to 10 5 ~10 6 a / mL;

[0031] (3) Mix 200-240 g of concentrated solution of carbonic anhydrase-producing bacteria and 4 g of yeast powder evenly, and add t...

Embodiment 1

[0036] (1) Sieve out 500 g of a certain type of natural clinoptilolite powder and 500 g of ceramsite with a particle size of 0.3 to 4.75 mm, wash them twice with tap water, and dry them for later use;

[0037] (2) Weigh 20g of sucrose, Na 2 HPO 4 12H 2 O5g, MgSO 4 1g, CaCO 3 2g, KCl0.2g, (NH 4 ) 2 SO 4 1g, 0.5g of yeast extract, dissolved in 2000mL of deionized water, adjusted to pH 7±0.5, prepared into a liquid medium, sterilized at 120°C for 20 minutes under high temperature regulation. Inoculate the Bacillus colioids into the cooled medium, and culture it with shaking at 30±0.5°C for 48 hours, and the shaking frequency is 170r / min. The above bacterial liquid was concentrated to 10 by centrifugation in a high-speed refrigerated centrifuge for 15 minutes. 5 ~10 6 pieces / mL, the rotation speed is 6000r / min;

[0038](3) Weigh 4g of yeast powder and 200g of the above-mentioned bacterial concentrate, mix them evenly on 400g of zeolite powder, and shake and cultivate the...

Embodiment 2

[0042] (1) Sieve 1000 g of ceramsite with a particle size of 0.3 to 4.75 mm, wash it twice with tap water, and dry it for later use;

[0043] (2) Weigh 60g of sucrose, NaNO 3 6g, MgSO 4 ·7H 2 O1g, KCl1g, FeSO 4 4H 2 O0.02g, K 2 HPO 4 2g, 30g of agar, dissolved in 2000mL of deionized water, adjusted to pH 6-6.5, prepared into a liquid medium, sterilized at 120°C for 20 minutes under high temperature regulation. Aspergillus niger was inoculated into the cooled medium, and cultured with shaking at 30±0.5°C for 36 hours at a shaking frequency of 170r / min. The above bacterial liquid was concentrated to 10 by centrifugation in a high-speed refrigerated centrifuge for 15 minutes. 5 ~10 6 pieces / mL, the rotation speed is 6000r / min;

[0044] (3) Weigh 4g of yeast powder and 200g of the above-mentioned bacterial concentrate, mix them evenly on 400g of ceramsite, and shake and cultivate them in a 30°C incubator for 6 hours, so that Aspergillus niger and yeast powder are fully im...

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Abstract

The invention provides a preparation method of a cement-based material having a deep self-repairing function. The preparation method disclosed by the invention comprises the following steps of: mixing concentrated solution of carbonic anhydrase generating bacteria with saccharomycete powder according to the mass ratio of (50-60) to 1, and immobilizing on a same carrier; mixing a substrate with a nutritional substance according to the mass ratio of (30-40) to 1, and fixing on another carrier; adding cement, sand and water by taking the carriers as lightweight aggregates, wherein the mass of the carriers is 17-23% of the total mass of mortar; uniformly stirring, and forming a test piece; and manufacturing a crack of the test piece after standard curing, and introducing air to maintain and repair under 25-30 DEG C constant-temperature water bath conditions. The repairing process of the test piece is monitored by utilizing a concrete nondestructive detection technology (ultrasonic sound); the repairing result is detected through a water permeability test; the result shows that the crack is completely repaired after repairing for 21 d; in addition, compared with other microbiological methods for repairing cracks of the cement-based material, the crack repairing speed and repairing depth are greatly improved.

Description

technical field [0001] The invention belongs to the interdisciplinary science and technology in the fields of microbiology and civil engineering materials, and relates to a preparation method of a cement-based material that uses microbial technology to rapidly self-repair cracks. Background technique [0002] Cement-based materials, especially concrete, due to long-term use and the influence of the surrounding complex environment, will produce local damage such as tiny cracks, which will reduce the service life of the structure in the slightest, and endanger the safety of the structure in severe cases. At present, the passive repair and later repair of cracks in cement-based materials mainly use reinforcement repair, anchoring, bonding, pouring and cathodic protection. The self-healing technologies of cement-based materials mainly include the following types: hollow fibers or capsules immobilize high molecular polymers with repairing properties; shape memory materials apply ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C04B28/00C04B14/02C04B14/04
Inventor 钱春香陈怀成任立夫罗勉
Owner SOUTHEAST UNIV
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