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Method for extracting gastrodin

A technology of gastrodin and gastrodia elata, which is applied in the field of extraction and purification of effective components of Chinese herbal medicines, can solve the problems of low extraction rate and low purity of gastrodin, and achieve the effects of easy operation, simple process, and easy control

Active Publication Date: 2015-01-07
神农架绿野食品开发有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the gastrodin obtained by the current technical method generally has the problems of low extraction rate (gastrodin yield) and low purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Grind the naturally dried Gastrodia elata, weigh 500g Gastrodia elata powder, and use 3000mL water: methanol: ethanol mixed solvent with a volume ratio of 4:1:1 to percolate for 24 hours under the action of 50W microwave, and apply the percolation solution to neutral Alumina chromatographic column, elute with 1200mL of 80% methanol solution, collect gastrodin section eluent, and load sample to macroporous resin column HPD500, elute with ethanol: ethyl acetate mixed solvent 1600mL of volume ratio 3:1, The eluate from the gastrodin segment was collected, rotary steamed and dried to obtain 1.67 g of gastrodin extract, the HPLC purity was 98.2%, and the gastrodin extraction rate was 0.328%.

Embodiment 2

[0032] Grind the naturally dried Gastrodia elata, weigh 500g Gastrodia elata powder, and percolate with 300mL of water: methanol: ethanol mixed solvent with a volume ratio of 5:1:1 under the action of 50W microwave for 24 hours, and apply the percolation solution to neutral Alumina chromatographic column, elute with 1500mL of 70% methanol solution, collect gastrodin segment eluent, and load sample to macroporous resin column HPD500, elute with ethanol: ethyl acetate mixed solvent 1500mL of volume ratio 4:1, Gastrodin segment eluate was collected, rotary steamed and dried to obtain 1.64 g of gastrodin extract, with HPLC purity of 98.0%, and gastrodin extraction rate of 0.323%.

Embodiment 3

[0034] Grind the naturally dried Gastrodia elata, weigh 500g Gastrodia elata powder, and use 3000mL water: methanol: ethanol mixed solvent with a volume ratio of 4:1:0.5 to percolate for 24 hours under the action of 50W microwave, and apply the percolation solution to neutral Alumina chromatographic column, elute with 1200mL of 80% methanol solution, collect gastrodin section eluent, and load sample to macroporous resin column HPD500, elute with ethanol: ethyl acetate mixed solvent 1600mL of volume ratio 3:1, The eluate from the gastrodin segment was collected, rotary evaporated, and dried to obtain 1.67 g of gastrodin extract, the HPLC purity was 98.5%, and the gastrodin extraction rate was 0.329%.

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Abstract

The invention discloses a method for extracting gastrodin. The method comprises the following steps: percolating gastrodia elata powder by a water-alcohol mixed solvent under the ultrasonic effect; loading percolation liquid to a neutral alumina chromatographic column, eluting by a methanol solution, and collecting gastrodin-section elution liquid; loading the percolation liquid to a macroporous resin chromatographic column, eluting by an alcohol-ester mixed solvent, and collecting gastrodin-section elution liquid; steaming, concentrating, and drying to obtain gastrodin extract. The gastrodin extract obtained by the method is high in purity, and the gastrodin extraction rate is high.

Description

technical field [0001] The invention relates to the field of extraction and purification of effective components of Chinese herbal medicines, in particular to a method for extracting gastrodin from Gastrodia elata. Background technique [0002] Gastrodia elata is an orchid plant, also known as Chijian, Limu, Guiduyou, Shencao, Duyaozhi, Chijianzhi, Dingfengcao, Helicao, Duyao, Automatic Grass or Water Artichoke, etc. The main component with higher content in Gastrodia elata is gastrodin (gasTCM LIBodin), also known as gastrodin, and its chemical composition is p-hydroxymethylphenyl-β-D-glucopyranoside (p-hydroxymethylphenyl-β-D-glucopyranoside ), has a good sedative and hypnotic effect, and has a relieving effect on neurasthenia, insomnia, and headache symptoms. [0003] Gastrodin can be extracted from Gastrodia elata or obtained by chemical synthesis. Up to now, there are many reports about extracting gastrodin from Gastrodia elata, and solvent extraction is generally use...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 顾万兴
Owner 神农架绿野食品开发有限责任公司
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