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Method for decoloring L-hydroxyproline fermentation broth by utilizing active carbon

A technology of hydroxyproline and activated carbon, applied in alkali metal oxides/hydroxides, inorganic chemistry, ion exchange, etc., can solve problems affecting separation and extraction, reduce the number of repeated regenerations, improve utilization, and save The effect of labor costs

Inactive Publication Date: 2015-01-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of L-hydroxyproline is mainly hydrolyzed gelatin method and fermentation method, but no matter which method of producing L-hydroxyproline is accompanied by the production of pigments, these pigments seriously affect the smooth progress of separation and extraction.
[0004] There is no relevant report about the decolorization of L-hydroxyproline fermentation broth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The specific decolorization method of continuous decolorization is carried out according to the following steps.

[0026] (1) The L-hydroxyproline fermentation broth was centrifuged in a high-speed desktop refrigerated centrifuge at 8000r / min for 20min to remove bacteria and solids, and the supernatant was taken and placed in a chromatographic cabinet at 4°C.

[0027] (2) Stir and clean the powdered activated carbon with deionized water, repeat several times, let it stand until the supernatant has no obvious impurities, then discard the deionized water and add twice the volume of 0.02mol / L hydrochloric acid, let it stand for two hours, and finally Wash with deionized water until nearly neutral, and dry in an oven.

[0028] (3) Weigh 4.0 g of dry powdered activated carbon, put it into a chromatography column, wash the column with hydrochloric acid with a pH value of about 4, ensure that there are no bubbles in the middle of the activated carbon, and then add the L-hydrox...

Embodiment 2

[0032] The specific decolorization method of continuous decolorization is carried out according to the following steps.

[0033] (1) The L-hydroxyproline fermentation broth was centrifuged in a high-speed desktop refrigerated centrifuge at 8000r / min for 20min to remove bacteria and solids, and the supernatant was taken and placed in a chromatographic cabinet at 4°C.

[0034] (2) Stir and clean the powdered activated carbon with deionized water, repeat several times, let it stand until the supernatant has no obvious impurities, then discard the deionized water and add twice the volume of 0.02mol / L hydrochloric acid, let it stand for two hours, and finally Wash with deionized water until nearly neutral, and dry in an oven.

[0035] (3) Weigh 6.0g of dry powdered activated carbon, put it into the chromatography column, wash the column with hydrochloric acid with a pH value of about 4, ensure that there are no bubbles in the middle of the activated carbon, and then add the L-hydro...

Embodiment 3

[0039] The specific decolorization method of continuous decolorization is carried out according to the following steps.

[0040] (1) The L-hydroxyproline fermentation broth was centrifuged in a high-speed desktop refrigerated centrifuge at 8000r / min for 20min to remove bacteria and solids, and the supernatant was taken and placed in a chromatographic cabinet at 4°C.

[0041] (2) The powdered activated carbon is stirred and cleaned with deionized water, repeated several times, and left standing until the supernatant has no obvious impurities, then discarding the deionized water and adding twice the volume of 0.02mol / L hydrochloric acid, standing for two hours, Finally, it was washed with deionized water until it was close to neutral, and dried in an oven.

[0042] (3) Weigh 8.0g of dry powdered activated carbon, put it into the chromatography column, wash the column with hydrochloric acid with a pH value of about 4, ensure that there are no bubbles in the middle of the activate...

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Abstract

The invention relates to a method for decoloring an L-hydroxyproline fermentation broth by utilizing active carbon. The method comprises pretreatment of active carbon, pretreatment of the fermentation broth, continuous decoloring of the L-hydroxyproline fermentation broth, regeneration of active carbon and other steps. According to the method, continuous decoloring is performed on the L-hydroxyproline fermentation broth by filling a chromatography column with powdered active carbon, and compared with a process of using powdered active carbon for decoloring in batches, active carbon can be repeatedly used for multiple times, column filling does not need repeatedly performing, regeneration can be directly performed after decoloring, the labor intensity is reduced, and the cost of purchasing active carbon is reduced. Additionally, the whole process is performed at room temperature, decoloring on a large scale is relatively easy to operate, high-temperature danger is avoided, energy is saved and cost is reduced. The method is mainly applied to removal of pigments in the L-hydroxyproline fermentation broth and is suitable for industrial application.

Description

technical field [0001] The invention relates to a method for decolorizing L-hydroxyproline fermentation broth by using activated carbon, which is suitable for the decolorization process of preparing L-hydroxyproline by fermentation. Background technique [0002] L-Hydroxyproline (Hyp) is a chiral molecule, which does not belong to the 20 common amino acids, and has 4 isomers in total. The hydroxyproline used in this experiment is trans-4-hydroxy-L-proline (trans-4-Hydroxy-L-Proline). L-hydroxyproline is widely distributed and is an important component of collagen, gelatin, plant cell wall proteins and microbial secondary metabolites. In recent years, L-hydroxyproline has played an irreplaceable role in food, medicine, cosmetics, etc. It can be used as a feed additive for infants and young children and some non-mammals. Blood pressure and other effects, in addition, it can also be used as an additive in cosmetics, with anti-oxidation and anti-radiation effects. [0003] Th...

Claims

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Application Information

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IPC IPC(8): C07D207/16B01J20/28B01D15/08
CPCC07D207/16
Inventor 张震宇袁春伟孔惠琳
Owner JIANGNAN UNIV
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