Method of detecting phenotypic character related genes in nuclear genome

A technology of nuclear genome and phenotypic traits, applied in the field of molecular biology, can solve the problems of no rapid detection of genes related to phenotypic traits

Inactive Publication Date: 2015-01-21
BEIJING FORESTRY UNIVERSITY
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  • Abstract
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Problems solved by technology

[0005] The present invention aims to provide a method for detecting genes related to phenotypic traits in the nuclear genome to solve the technical problem in the prior art that there is no method for rapidly detecting genes related to phenotypic traits in the nuclear genome

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  • Method of detecting phenotypic character related genes in nuclear genome
  • Method of detecting phenotypic character related genes in nuclear genome
  • Method of detecting phenotypic character related genes in nuclear genome

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Embodiment 1

[0037] 1) Measure the net photosynthesis of 1200 F2 populations of "YX01" (P.alba×P.glandulossa) crossed with "LM50" (P.tomentosa) in Xiaotangshan, Beijing (40°2′N, 115°50′E) rate, and select extreme individuals with net photosynthetic rate, uniform and repeated measurements, and screen 30 stable individuals with extreme net photosynthetic rate. Among them, there were 15 individuals with high net photosynthetic rate and 15 individuals with low net photosynthetic rate.

[0038] Among them, the measurement of photosynthetic efficiency is from 9:00 am to 11:00 am when the plants grow vigorously and the sun is sufficient. This time is more accurate for the measurement of photosynthesis. In order to ensure the accuracy of the determination of the net photosynthetic rate of the individuals used to build the pool, the inventors repeatedly measured the plants that showed high or low photosynthesis in the measurement on the same day.

[0039] 2) Use the Plant Qiagen RNAeasy kit to ext...

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Abstract

The invention discloses a method of detecting phenotypic character genes related in a nuclear genome. The method includes: S1) measuring a target phenotypic character of an F1-generation hybrid population and selecting an extreme target phenotypic character; S2) extracting RNA of the individuals with the extreme target phenotypic character, grouping by a BSA method, and constructing an expression gene chip; and S3) finding differentially expressed genes according to information of the expression gene chip, wherein the genes are the phenotypic character related genes in the nuclear genome. By application of the technical scheme, the target phenotypic character of the F1-generation hybrid population is screened by a plurality of times of measurement, the individuals with the stable extreme target phenotypic character are selected, mixing and grouping are performed by adoption of the BSA method and gene pools are constructed, thus highlighting differences of the target phenotypic character. A large number of genes are hybridized at the same time by combination of a gene chip technology. The differentially expressed genes related to the target phenotypic character are screened through analyzing chip results.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for detecting genes related to phenotype shape in nuclear genome. Background technique [0002] Photosynthesis is one of the most important chemical reactions in nature, and it is the basis of photosynthetic biomass. In forests, wood is converted from photosynthetic products. In agriculture, 90% of crop production comes from photosynthesis. Therefore, the research on photosynthesis has significance that cannot be ignored for food security caused by population growth and the development of new energy. [0003] With the in-depth study of photosynthesis, it is found that it consists of several complex processes, including light capture, carbon fixation, photophosphorylation, etc., and is related to nutrient absorption, transport efficiency, environmental factors, and biomass redistribution. Related, its regulatory network involves multiple levels, including nucle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6816C12Q2539/113
Inventor 张德强王博文杜庆章
Owner BEIJING FORESTRY UNIVERSITY
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