Tissue culture and rapid propagation method for eucalyptus urophydis DH32-26 varieties

A technology of tissue culture rapid propagation and variety, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of ineffective inheritance of good characteristics of parents, ineffective development of tissue culture work, and outstanding characteristics to be studied , to achieve the effects of improving economic and social benefits, being easy to promote and use, and reducing the risk of tissue culture

Active Publication Date: 2015-01-28
广西壮族自治区国有东门林场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0008] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be explored for the purposeful propagation of the excellent traits of excellent trees, and the tissue culture and rapid propagation methods that can be effectively popularized and applied. Due to the DH32-26 It is a perennial species of Eucalyptus grandis, and there are few tissue-cultured seedlings in the market, and resources are in short supply, but the tissue culture of this species has not been effectively carried out. The tissue culture research on the new species of Eucalyptus grandis DH32-26 is conducted, and the rapid propagation of this species , to meet the market supply, it is of great significance to improve the ecological and economic benefits of this variety

Method used

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  • Tissue culture and rapid propagation method for eucalyptus urophydis DH32-26 varieties

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Effect test

Embodiment 1

[0032] The method of tissue culture and rapid propagation of Eucalyptus urophylla DH32-26 variety is as follows:

[0033] ①The establishment of explants: select 4-5 years old, good trees with strong growth, thick trunks, no pests and diseases, cut them down from 20-25cm from the ground, and wait until the stump buds grow to 10cm, semi-lignified, and the bud eyes are not yet When sprouting, the sprouts are collected as explant material. In order to reduce the source of material pollution, when taking sprouts, it should be done after three consecutive days of fine weather. After removing most of the leaves from the harvested sprouts, rinse them with tap water for 15 minutes, soak them in 0.5% detergent solution for 15 minutes, and then rinse them with tap water.

[0034] ②Explant disinfection: Step ①The obtained explants are soaked in 75% alcohol for 30S, rinsed with sterile water for 5 times, drained, and then placed in a 0.1% mercury solution for 9 minutes, and rinsed with sterile...

Embodiment 2

[0041] The method of tissue culture and rapid propagation of Eucalyptus urophylla DH32-26 variety is as follows:

[0042] ①The establishment of explants: select 4-5 years old, good trees with strong growth, thick trunks, no pests and diseases, cut them down from 20-25cm from the ground, and wait until the stump buds grow to 10cm, semi-lignified, and the bud eyes are not yet When sprouting, the sprouts are collected as explant material. In order to reduce the source of material pollution, when taking sprouts, it should be done after three consecutive days of fine weather. After removing most of the leaves from the harvested sprouts, rinse them with tap water for 15 minutes, soak them in 0.5% detergent solution for 15 minutes, and then rinse them with tap water.

[0043] ②Explant disinfection: Step ①The obtained explants are soaked in 75% alcohol for 30 seconds, rinsed with sterile water 4 times, drained, and then placed in a 0.1% mercury liter solution for 9 minutes and rinsed with...

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Abstract

The invention discloses a tissue culture and rapid propagation method for eucalyptus urophydis DH32-26 varieties. The tissue culture and rapid propagation method is realized through the steps of explant establishing, explant disinfection, primary culture, subculture, rooting culture, domestication, transplanting and the like; a special culture medium is selected for primary culture, subculture and rooting culture, and reasonable domestication measures are adopted, so that the transplanted eucalyptus urophydis is rapid in growth and high in survival rate, and the aims of high propagation coefficient and good propagation effect are achieved. Meanwhile, the method disclosed by the invention is low in production cost, is an effective path for tissue culture and rapid propagation of a eucalyptus, and is wide in application range; and the genetic characters of the obtained tissue culture seedlings are consistent.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus macrocephalus DH32-26 variety. Background technique [0002] Eucalyptus grandis DH32-26 variety (E .urophylla×E . Grandis), It is Guangxi Dongmen Forest Farm, with Indonesian provenance Eucalyptus urophylla U16 (E ucalyptus urophylla ) as the female parent, and the Australian provenance Eucalyptus grandis G46 (E ucalyptus grandis ) is an excellent eucalyptus species obtained through vegetative propagation as a male parent hybrid dominant species. This species has fast growth and high yield, short rotation period (industrial fiber material); Straight, resistant to barrenness, and suitable for planting in large areas. Years of popularization tests have proved that the average tree height of the 5-year-old Eucalyptus grandis DH32-26 variety is 24.72m, the average diameter at breast height is 16.65cm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 苏勇陈德文梁庭辉沈云莫继有庞贞武黄全东吴兵梁秀莉陈允椿韦炳俭付淼吴满芬俸荣娣邓冬丽龚卫新
Owner 广西壮族自治区国有东门林场
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