Rapid determination method for coal-bed methanogen flora current activity and flora balance characteristic
A technology of methane flora and flora balance, which is applied in the directions of biochemical equipment and methods, and the determination/inspection of microorganisms to achieve the effects of improving accuracy, fast determination speed and high reliability
Inactive Publication Date: 2015-03-11
CHINA UNIV OF MINING & TECH
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Problems solved by technology
At present, there is no complete and feasible solution to complete the rapid and accurate identification and judgment of the current activity and balance characteristics of the coal seam methanogenic bacteria
Method used
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Embodiment
[0052] Example: figure 1 It is a schematic diagram of the measurement method and system composition of the current activity and balance degree characteristic determination technology of the coal seam methanogenic bacteria group. It lists the internal classification of the medium cultivation group and the coal-based cultivation group in the two cultivation systems, and describes the relationship between them.
[0053] figure 2 It is a flow chart of the activation and balance determination of coal seam methanogens, which introduces how the system completes the determination of the current activity of coal seam methanogens and how to determine the balance of the bacteria.
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A rapid determination method for coal-bed methanogen flora current activity and flora balance characteristic belongs to determination methods for coal-bed methanogen flora activity and balance. According to the determination method for coal-bed methanogen flora current activity and flora balance characteristic, synchronous testing is performed through functional medium culturing and coal-base medium culturing, wherein in functional medium culturing, controllable factors are utilized to determine the structure integrity and activated possibility of a to-be tested microflora; and in the coal-based medium culturing, an actual coal sample is utilized for performing culturing determination on bacterial strain so as to determine whether a to-be determined bacterial strain can be activated and cultured and can finish biogasification of coal by taking organic matters in specific coal as a basic carbon source in a real coal bed environment. Functional medium culturing results are used to provide reference parameters for the determination method, and coal-based medium culturing results are taken as variable parameters, so that the balance of the activated microbiota in the coal bed is obtained. The method is capable of realizing miniaturization of a testing system, and has the characteristics of being rapid in execution, fast in effectiveness and high in accuracy.
Description
technical field [0001] The invention relates to a method for judging the activity and balance degree of coal bed methane bacteria, in particular to a method for judging the characteristics of the current activity and balance degree of coal bed methanogenic bacteria. Background technique [0002] Coal seam biogas generation is an effective solution to solve the shortage of natural gas resources in my country and the imbalance between supply and demand. At the same time, the application of coal gasification is also an effective way to promote clean coal technology and reduce environmental pollution caused by coal combustion. At present, in the coal biogasification technology, the activity and integrity of the methanogenic bacteria in the coal seam are the key control factors that dominate and complete the gasification process. Whether the methanogenic flora in the target coal seam can be activated and the balance characteristics of the flora after activation are the leading c...
Claims
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IPC IPC(8): C12Q1/02
Inventor 肖栋萨蒂亚·哈波兰尼彭苏萍
Owner CHINA UNIV OF MINING & TECH
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