Hemifusus tuba microsatellite loci, and primers and application of hemifusus tuba microsatellite loci

A technology of microsatellite loci and snails, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve problems such as the destruction of natural resources of snails in sea areas

Inactive Publication Date: 2015-03-25
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the snails sold in the market are all caught in natural sea areas. However, due to the over-harvesting of snails and the deteriorating environmental pollution in recent years, the natural resources of snails in sea areas have been seriously damaged.

Method used

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  • Hemifusus tuba microsatellite loci, and primers and application of hemifusus tuba microsatellite loci
  • Hemifusus tuba microsatellite loci, and primers and application of hemifusus tuba microsatellite loci
  • Hemifusus tuba microsatellite loci, and primers and application of hemifusus tuba microsatellite loci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] 1. Screening of microsatellite loci

[0020] 1. Genomic DNA extraction

[0021] 1) Mince the snail gastropod muscle frozen at -80°C with a scalpel, quickly weigh about 100 mg and put it into a 1.5 ml sterilized centrifuge tube;

[0022] 2) Add 500 μL of CTAB lysis buffer and 10 μL of proteinase K (20 mg / ml) to each centrifuge tube, digest in a 56-degree water bath overnight, and mix well by turning over every few hours;

[0023] 3) After the sample is completely digested, add an equal amount of CIA (chloroform: isoamyl alcohol = 24:1), rotate for 20 minutes, centrifuge at 10,000 rpm for 10 minutes at room temperature, pipette the supernatant into another clean centrifuge tube (the middle layer cannot be sucked out) Repeat the CIA extraction once; 4) Add PCI (phenol: chloroform: isoamyl alcohol = 25:24:1), the same amount as the supernatant taken, rotate for 10 min, centrifuge at 10,000 rpm for 10 min at room temperature, take the supernatant and clean and sterilize it ...

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Abstract

The invention discloses 14 hemifusus tuba microsatellite loci and primers of the hemifusus tuba microsatellite loci. Research shows that amplification results of the primers have high polymorphism and stability and can be applied to detection of genetic diversity of populations, individual identification or molecular assisted breeding of hemifusus tuba. The hemifusus tuba microsatellite loci are applied to breeding conservation and scientific study of the hemifusus tuba; usable molecular markers are provided for studies on genetic diversity of the hemifusus tuba; a method for carrying out genetic individual identification on the hemifusus tuba by using the microsatellite loci is provided; the shortcomings of an existing basic theory are overcome; the genetic typing is carried out on PCR products of the microsatellite loci; the genetic typing results are used for analyzing wild individuals of the hemifusus tuba, namely, the individual identification of the hemifusus tuba can be realized.

Description

technical field [0001] The invention belongs to the technical field of DNA markers in molecular biology, and in particular relates to the microsatellite locus of picornis and its primer and application. Background technique [0002] Hemifusus tuba (Gmelin) belongs to Mollusca, Gastropoda, Neogastropoda, and Galeodidae. It is a warm-water species that lives in tropical and subtropical sea areas. Soft mud and sandy bottom with shallow water depth of 11-42m; the shell is relatively large, pear-shaped, hard and thick, and the surface of the shell is covered with yellowish brown or light brown fine and short fur; the height of the adult shell is 110 -200mm, shell width 70-117mm (about 150-300g), maximum shell height over 300mm (weight >500g); spiral layer about 8.5 layers, each layer grows faster in height and width, from the third layer of the spiral part to the The body whorl, each shoulder has a ring of nodules, and the body whorl becomes triangular spines. Pipe horn snai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/156
Inventor 潘英吴雪萍吴明灿周于娜苏翔驹
Owner GUANGXI UNIV
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