Color separation, bluing and transparentizing method for safranine-fast green counter staining procedure
A safranine and color separation technology, which is applied in the field of color separation, blueing and transparency, and can solve problems such as unreported processing of color separation, blueing and transparency.
Inactive Publication Date: 2015-03-25
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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For the process of paraffin sections of Elymus genus plants, refer to related reports, but there is no report on the specific treatment for its color separation, bluish transparency
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[0025] The present invention will be further described below in conjunction with the accompanying drawings.
[0026] Such as Figures 1 to 2 As shown, the old awn wheat formed a 32-cell oval proembryo 6 days after flowering (eg figure 1 -A). The proembryo cells further differentiated and formed rod-shaped embryos at 7 days after anthesis. At this time, the embryo development had entered the stage of differentiated embryos. The rod-shaped embryo has a distinct stalk (eg figure 1 -B). On the 8th day after flowering, a depression is formed, which is the growth point, and the upper part of the coleoptile and the scutellum begin to differentiate at the upper part of the growth point, and the scutellum and the coleoptile part begin to separate. This stage is called differentiation embryo I (Such as figure 1 -C). About 9 days after flowering, a depression appeared in the lower part of the growth point, at this time, the scutellum and the coleoptile separated, and the coleoptile...
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The invention discloses a color separation, bluing and transparentizing method for a safranine-fast green counter staining procedure. The method comprises the following steps: (1) putting a sarranine-dyed material in a 100mL 50% ethanol liquid containing 2mL concentrated HCl for 5s; (2) putting the material in a 100mL 95% ethanol liquid containing 2mL ammonia water for 1s; (3) putting the material in a 100mL absolute ethyl alcohol liquid for 3s; (4) dropping 2mL fast green dying liquid on the material for 1s; (5) putting the material in a mixed liquid containing 50mL xylene, 50mL absolute ethyl alcohol and 6mL clove oil for 1s; (6) putting the material in a mixed liquid containing 50mL xylene and 50mL absolute ethyl alcohol for 30s; (7) putting the material in a 100mL xylene liquid for 30s; and (8) putting the material in a 100mL xylene liquid for 30s. According to the color separation, bluing and transparentizing method for the safranine-fast green counter staining procedure, the specific time of color separation, bluing, fast-green counter staining, cleaning and transparentizing in the safranine-fast green counter staining procedure of Elymus L. plants by utilizing a paraffin method is determined.
Description
technical field [0001] The invention relates to a safranin-fast green counter-dyeing method for observing the embryonic development of Elymus genus plants, in particular to a method for color separation and bluing in the process of safranin-fast green counter-dyeing suitable for the embryonic development of Elymus genus plants . Background technique [0002] Elymus L. is the genus with the largest variety and the widest distribution in Triticeae, and it is an ancient wild grass species distributed in the cold temperate zone of the northern hemisphere. Elymus sibiricus is a model species of Elymus genus. It is an excellent forage grass that cattle, horses, and sheep like to eat. It is also an excellent perennial cultivated grass in northern my country. It is widely used in artificial grassland and Natural grassland improvement, as a close genus of wheat crops, is an important source of excellent genes for genetic improvement of wheat crops. [0003] Seeds are the genetic mat...
Claims
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IPC IPC(8): G01N1/30
Inventor 德英赵来喜穆怀彬高凤芹解继红王照兰徐春波
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI