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The method of color separation and bluing in the dyeing process of safranin-fast green

A technology of safranin and color separation, applied in the field of color separation, bluishization and transparency, can solve the problems of color separation, bluishization and transparency that have not been reported.

Inactive Publication Date: 2017-07-28
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the process of paraffin sections of Elymus genus plants, refer to related reports, but there is no report on the specific treatment for its color separation, bluish transparency

Method used

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  • The method of color separation and bluing in the dyeing process of safranin-fast green
  • The method of color separation and bluing in the dyeing process of safranin-fast green

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings.

[0026] Such as Figures 1 to 2 As shown, the old awn wheat formed a 32-cell oval proembryo 6 days after flowering (eg figure 1 -A). The proembryo cells further differentiated and formed rod-shaped embryos at 7 days after anthesis. At this time, the embryo development had entered the stage of differentiated embryos. The rod-shaped embryo has a distinct stalk (eg figure 1 -B). On the 8th day after flowering, a depression is formed, which is the growth point, and the upper part of the coleoptile and the scutellum begin to differentiate at the upper part of the growth point, and the scutellum and the coleoptile part begin to separate. This stage is called differentiation embryo I (Such as figure 1 -C). About 9 days after flowering, a depression appeared in the lower part of the growth point, at this time, the scutellum and the coleoptile separated, and the coleoptile...

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Abstract

The invention discloses a method for color separation and bluing in the process of safranin-fast green counter-dyeing. The process is as follows: (1) placing the safranin-dyed material in 100mL of 50% ethanol solution containing 2mL of concentrated HCl 5s; (2) Place in 100mL 95% ethanol solution containing 2mL ammonia water for 1s; (3) Place in 100mL absolute ethanol solution for 3s; (4) Drop 2mL fast green staining liquid on top of the material for 1s; (5 ) in a mixture of 50mL xylene + 50mL absolute ethanol + 6mL clove oil for 1s; (6) in a mixture of 50mL xylene + 50mL absolute ethanol for 30s; (7) in 100mL xylene solution for 30s (8) placed in 100mL xylene solution for 30s. This method determines the specific time of color separation, blueing, fast green counterstaining, cleaning and transparency in the process of safranin-fast green counter-dyeing of Elymus genus paraffin section method.

Description

technical field [0001] The invention relates to a safranin-fast green counter-dyeing method for observing the embryonic development of Elymus genus plants, in particular to a method for color separation and bluing in the process of safranin-fast green counter-dyeing suitable for the embryonic development of Elymus genus plants . Background technique [0002] Elymus L. is the genus with the largest variety and the widest distribution in Triticeae, and it is an ancient wild grass species distributed in the cold temperate zone of the northern hemisphere. Elymus sibiricus is a model species of Elymus genus. It is an excellent forage grass that cattle, horses, and sheep like to eat. It is also an excellent perennial cultivated grass in northern my country. It is widely used in artificial grassland and Natural grassland improvement, as a close genus of wheat crops, is an important source of excellent genes for genetic improvement of wheat crops. [0003] Seeds are the genetic mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 德英赵来喜穆怀彬高凤芹解继红王照兰徐春波
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI