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Method for measuring object to be measured

A measurement method and a technology for measuring objects, which are applied in the directions of measuring devices, scattering characteristic measurement, electromagnetic wave re-radiation, etc., can solve problems such as increased operating procedures and deviations in measurement results, and achieve the effect of simple procedures

Inactive Publication Date: 2015-03-25
MURATA MFG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, a separate process of extracting the object to be measured is required before the measurement, and there is a problem that the number of work steps for the measurement increases.
[0005] In addition, for example, when using a membrane filter or the like to filter and extract an analyte from a sample such as a liquid or a gas, it is necessary to transfer the extracted analyte to a void-arranged structure by transfer or the like. All the extracted objects to be measured are moved to the void arrangement structure, so there may be large deviations in the measurement results

Method used

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  • Method for measuring object to be measured

Examples

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Embodiment 1

[0069] In this example, yeast was filtered and extracted from the sample using the void-arranged structure, and electromagnetic waves were directly irradiated to the void-arranged structure to which the yeast was attached, thereby measuring the number of yeast in the sample. Hereinafter, the details thereof will be described.

[0070] First, a culture solution in which yeast having an average cell diameter of 5 μm was cultured was prepared. After washing the culture solution twice with pure water by the centrifugal precipitation method, pure water was added to the precipitate (yeast) and mixed to obtain a yeast suspension.

[0071] For the obtained yeast suspension, methylene blue staining was carried out, and after the dead yeast was stained, the number of viable cells in the aqueous solution was measured using an automatic cell counting device (Cellometer (registered trademark) Nexcelom Bioscience Company), and the result was 5 × 10 7 [piece / mL].

[0072] The yeast suspens...

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Abstract

The present invention is a method for measuring the amount or presence of an object to be measured within a specimen, the method being characterized by involving: a filtration step in which the object to be measured is filtered from the specimen by using, as a physical filter, a void-arranged structural body having multiple voids penetrating in a direction that is perpendicular to a main surface, and in which the object to be measured is held by the void-arranged structural body; and a measurement step in which the void-arranged structural body holding the object to be measured is irradiated with electromagnetic waves and in which the property of the electromagnetic waves that scattered at the void-arranged structural body is detected.

Description

technical field [0001] The present invention relates to a method for measuring an object to be measured. More specifically, it relates to measuring the object to be measured by holding an object to be measured in a void arrangement structure having a void, irradiating the void arrangement structure with electromagnetic waves, and detecting the characteristics of the electromagnetic waves scattered by the void arrangement structure. Determination method of presence or quantity. Background technique [0002] Conventionally, in order to analyze the characteristics of a substance, a measurement method has been employed in which an object to be measured is held in a void arrangement structure, an electromagnetic wave is irradiated to the void arrangement structure holding the object to be measured, and the transmission spectrum thereof is analyzed to detect the measured object. The presence or quantity of a thing. Specifically, for example, a method of analyzing a transmission ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/03G01N21/47G01S17/87G01S17/04
CPCG01N21/03G01N21/3581G01N21/4788G01N2021/0339G01S17/04G01S17/87
Inventor 神波诚治近藤孝志白井伸明冈田俊树长谷川慎
Owner MURATA MFG CO LTD