Method for measuring object to be measured
A measurement method and a technology for measuring objects, which are applied in the directions of measuring devices, scattering characteristic measurement, electromagnetic wave re-radiation, etc., can solve problems such as increased operating procedures and deviations in measurement results, and achieve the effect of simple procedures
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[0069] In this example, yeast was filtered and extracted from the sample using the void-arranged structure, and electromagnetic waves were directly irradiated to the void-arranged structure to which the yeast was attached, thereby measuring the number of yeast in the sample. Hereinafter, the details thereof will be described.
[0070] First, a culture solution in which yeast having an average cell diameter of 5 μm was cultured was prepared. After washing the culture solution twice with pure water by the centrifugal precipitation method, pure water was added to the precipitate (yeast) and mixed to obtain a yeast suspension.
[0071] For the obtained yeast suspension, methylene blue staining was carried out, and after the dead yeast was stained, the number of viable cells in the aqueous solution was measured using an automatic cell counting device (Cellometer (registered trademark) Nexcelom Bioscience Company), and the result was 5 × 10 7 [piece / mL].
[0072] The yeast suspens...
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