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A kind of method of water lily tissue culture

A technology of tissue culture and water lily, applied in the field of plant tissue culture, can solve problems such as no successful examples

Active Publication Date: 2016-01-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on tissue culture research of water lily before this, and there is no successful example

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] The invention provides a kind of method for water lily tissue culture, and its steps are:

[0039] 1), the preparation of culture medium

[0040] (1) Basic culture medium: adopt solid-liquid double-layer culture medium, the upper layer is sterile distilled water; the lower layer is MS solid medium, in which sucrose is 20-30g / L, agar is 5-9g / L, pH=5.8;

[0041] (2) Induction medium solid layer: MS+TDZ2.0mg / L+NAA0.5mg / L+activated carbon 0.2mg / L;

[0042] (3) Proliferation medium solid layer: MS+6-BA3.0mg / L+NAA0.2mg / L;

[0043] (4) Solid layer of rooting medium: MS+NAA0.1mg / L+IBA0.1mg / L;

[0044] 2), selection and sterilization of explants

[0045] Dig water lily rhizomes and remove the leaves, cut off the terminal buds, side buds and part of the rhizome tissue, cut off the leaves and flocs around the buds with a sharp blade, trim and smooth, soak in saturated detergent solution for 60 minutes, and then rinse with tap water Clean, then soak in alcohol with a volume rat...

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Abstract

The invention discloses a method for tissue culture of water lilies, and the specific operation steps are as follows: 1) preparation of a culture medium, including basic culture medium and components of the culture medium at each stage of tissue culture; 2) selection and disinfection of explants; 3) ) induction and proliferation of adventitious buds; 4) rooting culture; 5) transplanting. Beneficial effects of the present invention: the present invention utilizes plant tissue culture technology to induce and proliferate water lily buds, and can obtain a large number of high-quality strong seedlings with consistent genetic traits in a short period of time, overcoming the shortcoming of slow conventional propagation methods, and simultaneously Large-scale production and preservation of germplasm resources are of positive significance.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for water lily tissue culture. Background technique [0002] Water lily (Nymphaeate tragona Georgi) is a perennial aquatic herb of the family Nymphaeaceae, with beautiful flowers and leaves, rich colors, long flowering period, and high ornamental value; the roots of water lilies can absorb toxic substances such as lead, mercury, and phenol in water, which is rare Water purification plants; in addition, water lilies also have certain medicinal value. However, water lilies mostly rely on ramet propagation and sowing propagation, with low reproduction coefficient and long cycle, which are difficult to meet the needs of the market. Therefore, the rapid propagation of water lilies using plant tissue culture technology can obtain a large number of seedlings with the same genetic traits as the mother plant in a short period of time, which is of great significance for preservi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王燕汪一婷吕永平牟豪杰陈志陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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