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A kind of method for aseptic seeding and regeneration system establishment of black feather jade

A technology of aseptic seeding and system, applied in the field of black feather jade, can solve problems such as unsuccessful examples

Active Publication Date: 2018-05-08
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the establishment of a tissue culture regeneration system for black feather jade before this, and there is no successful example

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The invention provides a method for aseptic seeding and regeneration system establishment of black feather jade, the steps of which are:

[0023] 1), the preparation of culture medium

[0024] (1) Basic medium: MS or 1 / 2MS, in which sucrose 30g / L, agar 7g / L, pH=5.8;

[0025] (2) Induction medium: MS+6-BA 0.25~0.5mg / L+NAA 0.01~0.05mg / L;

[0026] (3) Differentiation medium: MS+6-BA 0.01~0.05mg / L;

[0027] (4) Proliferation medium: MS+6-BA 0.05~0.2mg / L+NAA 0.01~0.05mg / L;

[0028] (5) Strong seedling rooting medium: MS+6-BA 0.01~0.05mg / L+NAA 0.1~0.3mg / L;

[0029] 2), disinfection and inoculation of seeds

[0030] Take the seeds of black feather jade as the explant material, put it in a 1.5ml or 2ml PE centrifuge tube for disinfection treatment, first soak it in detergent solution for 0.5h, then wash it with tap water, and then adjust the volume ratio concentration Soak in 70% alcohol and 1% sodium hypochlorite solution for 50 seconds and 6 minutes, respectively, and fi...

Embodiment 2

[0038] The invention provides a method for aseptic seeding and regeneration system establishment of black feather jade, the steps of which are:

[0039] 1), the preparation of culture medium

[0040] (1) Basic medium: MS medium, in which sucrose 20-30g / L, agar 6-9g / L, pH=5.8;

[0041] (2) Induction medium: MS+6-BA 0.2mg / L+NAA 0.05mg / L;

[0042] (3) Differentiation medium: MS+6-BA 0.05mg / L;

[0043] (4) Proliferation medium: MS+6-BA 0.1mg / L+NAA 0.02mg / L;

[0044] (5) Strong seedling rooting medium: MS+6-BA 0.05mg / L+NAA 0.2mg / L;

[0045] 2), disinfection and inoculation of seeds

[0046] Take the seeds of black feather jade as the explant material, put it in a 1.5ml or 2ml PE centrifuge tube for disinfection treatment, first soak it in detergent solution for 0.5h, then wash it with tap water, and then adjust the volume ratio concentration Soak in 70% alcohol and 1% sodium hypochlorite solution for 50 seconds and 6 minutes, respectively, and finally rinse with sterile water ...

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Abstract

The invention discloses a method for aseptic seeding and regeneration system establishment of black feather jade. The specific operation steps are as follows: 1) preparation of culture medium, including basic culture medium and components of culture medium in each stage of tissue culture; 2) seed disinfection and inoculation; 3) induction and differentiation of callus; 4) proliferation of adventitious bulbs; 5) rooting culture of strong seedlings. The present invention utilizes plant tissue culture technology to carry out aseptic sowing and regeneration rapid propagation culture to black feather jade, can obtain a large amount of high-quality strong seedlings with consistent genetic traits in a short time, overcomes the shortcoming of conventional propagation method slowness, and its germplasm resources Collection and preservation and large-scale production all have positive significance.

Description

technical field [0001] The invention relates to the technical field of black feather jade, in particular to a method for aseptic sowing and regeneration system establishment of black feather jade. Background technique [0002] Lophophora williamsii (Lophophora williamsii) is a plant of the genus Lophophora williamsii in the cactus family. It is native to northern Mexico. It has a unique shape and is one of the famous representative species of popular small ornamental succulents. In addition, black feather jade also has important medicinal value, and has a significant effect on the treatment of asthma, lung cancer and mental illness. However, the growth rate of black feather jade is slow, the natural reproduction rate is low, coupled with the over-exploitation of human beings and the destruction of its original living environment, the current number of black feather jade is small, and the price is high, which makes its popularization and application very difficult. big limit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 王燕汪一婷吕永平牟豪杰陈剑平陈志
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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