Regeneration Method of Sleeping Bag Tissue Culture

Abstract

The invention discloses a tissue culture regeneration method of Gerradanthus macrorrhizus. The method comprises steps as follows: 1), Gerradanthus macrorrhizus seeds are disinfected, then the disinfected Gerradanthus macrorrhizus seeds are inoculated into a basal culture medium and subjected to basal culture, and aseptic seedlings are obtained; 2), cotyledons of the aseptic seedlings are cut into sections and inoculated into an induction culture medium for induction culture, and adventitious buds are obtained; 3), the adventitious buds are cut off and inoculated into a proliferation culture medium for proliferation culture; 4), the adventitious buds after proliferation culture are transferred into a rooting culture medium for rooting culture, and Gerradanthus macrorrhizus plants are obtained; culture conditions of the steps 1)-4) at least meet following requirements: the culture is sterile and breathable, the basal culture medium is an MS culture medium, the induction culture medium is a 6-BA-containing MS culture medium, the proliferation culture medium contains the MS culture medium, and the rooting culture medium contains 1 / 2 MS culture medium. With the adoption of the method, high-quality adult seedlings of the Gerradanthus macrorrhizus can be obtained rapidly.

Description

technical field [0001] The invention relates to tissue culture of sleeping bag, in particular to a regeneration method of tissue culture of sleeping bag. Background technique [0002] Sleeping bag (Gerradanthus macrorrhizus), a famous succulent bulb ornamental plant of Cucurbitaceae (Cucurbitaceae) sleeping bag genus (Gerradanthus), native to East Africa. In 2005, the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province began to introduce sleeping bag seedlings from Africa for ground planting and domestication and achieved success. The perennial sleeping bag plant has attracted much attention among succulents with its huge bulbs and graceful posture. [0003] Sleeping bag cultivation is mainly based on conventional propagation methods, including sowing, cutting propagation, etc. According to existing reports, there are currently problems such as low reproduction coefficient, limited efficiency, and susceptibility to environmental factors. Contents of the inv...

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Problems solved by technology

[0003] Sleeping bag cultivation is mainly based on conventional propagation methods, including sowing, cutting propagation, etc. According to existing reports, there are currently problems such as low reproduction coefficient, limited efficiency, and susceptibility to environmental factors.

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