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Method for establishing tissue culture regeneration system of tylecodon paniculatus

A kind of apangong, tissue culture technology, applied in the field of plant shoot tip culture, plant tissue culture technology, can solve the problems of unsuccessful examples and so on

Active Publication Date: 2015-06-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, before this, there was no report on the establishment of the tissue culture regeneration system of Afang Gongshou, let alone any successful examples

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The invention provides a method for establishing the tissue culture regeneration system of Abang Gongshou, the steps of which are as follows:

[0031] 1) Preparation of culture medium

[0032] (1) Basic medium: MS medium, in which sucrose is 20-30g / L, agar is 5-9g / L, pH=5.8;

[0033] (2) Induction medium: MS+6-BA4~8mg / L+NAA0.05~0.5mg / L;

[0034] (3) Differentiation medium: MS+6-BA0.05~0.5mg / L+NAA0.05~0.5mg / L;

[0035] (4) Proliferation medium: MS+6-BA0.5~1.0mg / L+NAA0.05~0.2mg / L;

[0036] (5) Strong seedling medium: MS+6-BA0.05~0.1mg / L+NAA0.05~0.1mg / L;

[0037] 2) Selection and sterilization of explants

[0038] Take the young inflorescences of Efang Gongshou as the explant material, remove the outer covering of the closed flower buds on the inflorescences, and then cut off the flower buds and some scapes one by one, soak them in concentrated washing powder solution for 10-60 minutes, and then rinse them with tap water , and then soaked in alcohol with a volume rati...

Embodiment 2

[0048] The invention provides a method for establishing the tissue culture regeneration system of Abang Gongshou, the steps of which are as follows:

[0049] 1) Preparation of culture medium

[0050] (1) Basic medium: MS medium, in which sucrose is 20-30g / L, agar is 5-9g / L, pH=5.8;

[0051] (2) Induction medium: MS+6-BA6mg / L+NAA0.5mg / L;

[0052] (3) Differentiation medium: MS+6-BA0.2mg / L+NAA0.2mg / L;

[0053] (4) Proliferation medium: MS+6-BA0.5mg / L+NAA0.05mg / L;

[0054] (5) Strong seedling medium: MS+6-BA0.05mg / L+NAA0.05mg / L;

[0055] 2) Selection and sterilization of explants

[0056] Take the young inflorescences of Efang Gongshou as the explant material, remove the outer covering of the closed flower buds on the inflorescences, and then cut off the flower buds and some scapes one by one, soak them in concentrated washing powder solution for 10-60 minutes, and then rinse them with tap water , and then soaked in alcohol with a volume ratio of 70% and sodium hypochlorite so...

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Abstract

The invention discloses a method for establishing a tissue culture regeneration system of tylecodon paniculatus. The method specifically comprises the operation steps of: (1) preparing a culture medium, including a basic culture medium and components of the culture medium adopted in each stage of the tissue culture; (2) selecting and sterilizing explants; (3) inducing and breeding the callus; (4) differentiating and breeding adventitious buds; (5) culturing strong seedlings; and (6) transplanting. According to the method, the tylecodon paniculatus is subjected to the regeneration culture by utilizing the plant tissue culture technology, and a large number of excellent-quality strong seedlings with the consistent inheritable characters can be gained in a short time, so that the shortcoming of low speed of the general breeding method is overcome, the positive significance is realized in massive production and storage of germplasm resources; and the technology also provides solid experimental foundation for the establishment of a genetic transformation system.

Description

technical field [0001] The invention relates to plant tissue culture technology and plant shoot tip culture technology, and mainly relates to a method for establishing a tissue culture regeneration system of Abang Gongshou. Background technique [0002] Abang Gongshou is a succulent plant of the genus Haworthia in the Liliaceae family. Its leaves are thick and juicy, its windows are crystal clear and transparent, and it has a city wall texture, which is regular and beautiful. It is an excellent succulent plant variety. Due to its low natural reproduction rate, and its origin is not in my country, coupled with excessive human excavation and damage to its habitat, the current quantity is small and the market price remains high, which limits its promotion. Therefore, the rapid propagation of Afang Gongshou by plant tissue culture technology is of great significance for the preservation of excellent germplasm resources and the propagation of famous and rare varieties, so as to r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王燕陈剑平汪一婷牟豪杰吕永平陈志
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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