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Tissue culture and rapid propagation method of cyrtanthus saturn

A technology of tissue culture rapid propagation and vertical tube flower, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as no successful examples, achieve short rooting time, simple and easy disinfection method, fast The effect of short cycle time

Active Publication Date: 2021-04-09
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But before this, also do not have the research report about the tissue culture fast multiplication of weeping tube flower, let alone successful example

Method used

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  • Tissue culture and rapid propagation method of cyrtanthus saturn
  • Tissue culture and rapid propagation method of cyrtanthus saturn
  • Tissue culture and rapid propagation method of cyrtanthus saturn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1, a kind of method for tissue culture rapid propagation of vertical tube flower:

[0086] Step 1, the preparation of culture medium:

[0087] Induction medium: MS+6-BA 1.0mg / L+NAA 0.01mg / L;

[0088] Proliferation medium: MS+6-BA 0.5mg / L+NAA 0.02mg / L;

[0089] Rooting medium: 1 / 2MS+NAA 0.1mg / L+IBA 0.1mg / L;

[0090] All the other are the same as "method 1, a kind of tissue culture and rapid propagation method of weeping tube flower", and the obtained results are as follows:

[0091] Step 3, induction of adventitious buds: after about 3 weeks, adventitious bud clusters can be induced; the induction rate of adventitious buds is 100%;

[0092] Step 4, proliferation and subculture of adventitious buds: the proliferation coefficient was 3.17 when cultured for 4 weeks. Subculture every 4 weeks.

[0093] Step 5, rooting induction: the rooting culture time is 8 days; at this time, the root primordia are induced to form.

[0094] Step 6, harden the seedlings for ...

Embodiment 2-7

[0095] Embodiment 2-7, a method for tissue culture and rapid propagation of T. chinensis, the difference from Example 1 is as described in Table 1 below.

[0096] Table 1

[0097]

Embodiment 8-13

[0098] Embodiment 8-13, a kind of tissue culture rapid propagation method of weeping tube flower, and the difference of embodiment 1 is described in the following table 2.

[0099] Table 2

[0100]

[0101]

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method of cyrtanthus saturn. The method comprises the following steps of setting a culture medium; selecting and disinfecting explants, and ensuring that outer scales have no defects; carrying out adventitious bud induction culture on disinfected bulbil in an induction culture medium; carrying out adventitious bud proliferation on an adventitious bud cluster generated by induction, segmenting the adventitious bud cluster obtained by proliferation into single plants under a sterile condition, and then transferring the single plants to a rooting culture medium for rooting induction culture; and performing transplanting and seedling hardening on the cyrtanthus saturn plants after rooting induction. According to the method, a large number of cyrtanthus saturn high-quality strong seedlings with consistent inheritable characters can be obtained in a short time.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of weeping tube flowers. Background technique [0002] Cyrtanthus spp. is a perennial bulbous plant of the genus Cyrtanthus in the family Amaryllidaceae, also known as Cyrtanthus spp. It blooms in winter and spring. It has a unique flower shape, a long flowering period, and evergreen leaves. It is an excellent potted ornamental flower. Its flowers have a sweet fragrance, and the flower stems are slender and elegant and upright. It can also be used as a high-grade cut flower material. [0003] Since the pendant flower is native to Africa, its market price is relatively high, especially some rare varieties are even more expensive. Weeping tube flowers mainly rely on ramet propagation, but the reproductive efficiency is low, the cycle is long and limited by the amount of female parent material, it is difficult to meet the needs of the market. Theoretically, using plant tissue cu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李海营王燕汪一婷吕永平牟豪杰陈志陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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