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Trypsin digestive juice, preparation method and ready-to-use phlegm digesting device

A technology of trypsin and digestive juice, which is applied in the field of ready-to-use sputum digestion device, preparation, and trypsin digestive juice. It can solve the problems of heavy workload, time-consuming, and poor stability of the mixed solution, so as to reduce the workload, time and process. Convenient, quick and cost-saving effect

Active Publication Date: 2015-04-22
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method will inevitably increase the storage cost of reagents, but also has the problems of heavy workload, low efficiency, and time-consuming, and the stability of the mixed solution is poor.
[0006] The trypsin commercial reagent using the second method above even needs to be rethawed (liquid A needs to be frozen at -20°C), and cannot maintain high activity for a long time at room temperature, so it needs to be stored at low temperature, which greatly increases the operating time and cost
[0007] The above methods of using trypsin sputum digestion solution all have problems such as too many steps and cumbersome operations, which greatly increase the doctor's time cost and storage cost

Method used

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  • Trypsin digestive juice, preparation method and ready-to-use phlegm digesting device
  • Trypsin digestive juice, preparation method and ready-to-use phlegm digesting device
  • Trypsin digestive juice, preparation method and ready-to-use phlegm digesting device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Formulation:

[0038]

[0039] 2. Process steps

[0040] (1) Taking materials: get qualified HEPES, NaOH, NaCl, dextrin, trypsin and purified water according to the above formula;

[0041] (2) Preparation: first take 60% (v / v) purified water for preparation, add HEPES, stir and mix, and adjust the pH to 6.50 with NaOH;

[0042] (3) Add NaCl and dextrin, stir and dissolve, and filter;

[0043] (4) Add trypsin, stir to dissolve, add the remaining purified water to make up the volume of sputum digestive juice to 1 L, and filter through filter membranes with pore diameters of 0.45 μm and 0.22 μm to obtain sputum digestive juice.

[0044] 3. Performance testing

[0045] Using benzoyl L-phenylarginine ethyl ester (English as benzoyl L-arginine ethyl ester, abbreviated as BAEE) as the substrate, it was determined by ultraviolet absorption method. The ultraviolet absorption of benzoyl L-arginine ethyl ester at a wavelength of 253nm is far weaker than that of benzoyl L...

Embodiment 2

[0053] 1. Formulation:

[0054]

[0055] 2. Process steps

[0056] (1) Feed: Take qualified MES, NaOH, MgCl according to the above formula 2 , trehalose, trypsin and purified water;

[0057] (2) Preparation: First take 60% (v / v) purified water for preparation, add MES, stir and mix well, adjust the pH to 7.50 with KOH,

[0058] (3) Add MgCl 2 and trehalose, stir to dissolve, and filter;

[0059] (4) Add trypsin, stir to dissolve, add the remaining purified water to make up the volume of sputum digestive juice to 1 L, and filter through filter membranes with pore diameters of 0.45 μm and 0.22 μm to obtain sputum digestive juice.

[0060] 3. Performance testing

[0061] The absorbance change value and trypsin activity of the above-mentioned sputum digestive juice were tested by the same method as in Example 1, and recorded in Table 2 below.

[0062] Divide a sputum sample into two groups, one group is digested with the sputum digestive juice prepared above as the experi...

Embodiment 3

[0066] 1. Formulation:

[0067]

[0068] 2. Process steps

[0069] (1) Feed: Take qualified TAPS, NaOH, MgCl according to the above formula 2 , NaCl, BSA, trypsin and purified water;

[0070] (2) Preparation: first take 60% (v / v) purified water for preparation, add TAPS, stir and mix, and adjust the pH to 8.00 with NaOH;

[0071] (3) Add MgCl 2 , NaCl and BSA, stir to dissolve, and filter;

[0072] (4) Add trypsin, stir to dissolve, add the remaining purified water to make up the volume of sputum digestive juice to 1 L, and filter through filter membranes with pore diameters of 0.45 μm and 0.22 μm to obtain sputum digestive juice.

[0073] 3. Performance testing

[0074] The absorbance change value and trypsin activity of the above-mentioned sputum digestive juice were tested by the same method as in Example 1, and recorded in Table 3 below.

[0075] Divide a sputum sample into two groups, one group is digested with the sputum digestive juice prepared above as the exp...

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PUM

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Abstract

The invention discloses trypsin digestive juice, a preparation method and a ready-to-use phlegm digesting device. The trypsin digestive juice comprises the main components: trypsin and a solution stabilizer, wherein the solution stabilizer is selected from at least one of the following substances: dextrin, mycose and bovine serum albumin. The trypsin digestive juice disclosed by the invention not only can be stably stored under a working pH condition, but also can be stably stored at room temperature and working concentration, so that the storage cost is greatly lowered, and the trypsin digestive juice is ready-to-use and convenient and efficient.

Description

technical field [0001] The invention belongs to the field of sputum digestion liquid, and in particular relates to a trypsin digestion liquid, a preparation method and a ready-to-use sputum digestion device. Background technique [0002] Sputum Digestion Solution is a reagent used to digest sputum specimens and liquefy the sputum. As an auxiliary reagent, the main purpose is to liquefy and homogenize the sputum specimen, so that some pathogenic bacteria with low content and wrapped in mucin can be released into the solution, so as to improve the detection rate of pathogenic bacteria and make the test Physicians can have a better detection effect in the process of sputum smear and sputum culture. [0003] At present, there are mainly two ways to digest sputum clinically: 1. Compounds that act on protein disulfide bonds and have reducing properties, such as: dithiothreitol (DTT), N-acetylcysteine ​​(NAC), Tris(2-carboxyethyl)phosphine (TCEP), β-mercaptoethanol, etc.; 2. Enzy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N1/20
CPCC12N9/6427C12N9/96C12Y304/21004
Inventor 林军岳徐建建蒲建文
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD
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