An anti-wrong sample addition device for ninety-six-well pcr plate

Abstract

The invention discloses a sample adding error preventing device for a ninety-six-hole PCR plate. The sample adding error preventing device comprises a ninety-six-hole PCR plate, a locating sliding seat, a base, a lead screw nut unit, a motor and a counter-emitting photoelectric sensor, wherein the ninety-six-hole PCR plate is fixedly connected with the locating sliding seat to form an integer; a rail foot arranged at the bottom of the locating sliding seat is matched with a guide rail on the base; and a lead screw nut is connected with the base and the locating sliding seat. An encoder contained in the motor is connected with the right end of a lead screw; a transmitter and a receiver of the counter-emitting photoelectric sensor are located at outer sides of the front and rear end faces of the locating sliding seat; a counter-emitting connecting line of the transmitter and the receiver is superposed with a central connecting line of a column of holes in the top left side of the ninety-six-hole PCR plate when the locating sliding seat is located on an initial position relative to the base; and a sensor switch is connected with the encoder. Only a sample adding row index is needed to be remembered in the sample adding operation process, so that the memory capacitance is reduced, the mental burden is relieved, the sample adding confusion phenomenon is reduced, the operation efficiency is increased, and the sample adding redoing probability, time cost and unnecessary experimental material wastes are reduced.

Description

technical field [0001] The invention relates to the related field of 96-well PCR plate, in particular to an auxiliary device which can effectively avoid adding wrong samples to each well involved in the process of adding samples to the 96-well PCR plate. Background technique [0002] Ninety-six-well PCR plates are the most common consumables in molecular biology laboratories. They are used as primers, TaqDNA polymerase, dNTP, Mg 2+ , buffer, template nucleic acid, etc. PCR plates are widely used in the fields of genetics, biochemistry, immunity, and medicine. They are not only used in basic research such as gene isolation, cloning, and nucleic acid sequence analysis, but also in the diagnosis of diseases or wherever there is DNA or RNA. In general, the operator will put the required primers, TaqDNA polymerase, dNTP, Mg 2+ , buffer solution and other components (except the nucleic acid template) are calculated and mixed evenly before dispensing, and finally different nuclei...

AI Technical Summary

Problems solved by technology

During the process of adding samples, the operator needs to concentrate on accurately memorizing the row and column position of the hole that needs to be added next, which creates a heavy mental burden on the operator, and the operator is prone to confusion in adding samples under long-term operation. In order to avoid adding wrong samples, the operator needs to record the hole positions that have been added, so as to check when the memory is confused. During this process, the operator often has to look back at the corresponding letters and numbers many times. The probability of sample addition errors is still high, resulting in a high probability of experiment redoing, resulting in a waste of time and experimental consumables

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