Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer

A technology of nucleic acid aptamer and estradiol, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of complex sample pretreatment, etc., and overcome the relatively complicated electrochemical operation, simple operation, and good selectivity Effect

Inactive Publication Date: 2015-05-06
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0004] Aiming at the defects in the prior art, the purpose of the present invention is to overcome the shortcomings and deficiencies of traditional large-scale instrument detection of estradiol requiring complex pretreatment of samples and requiring professio

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  • Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer
  • Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer
  • Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer

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Embodiment Construction

[0036] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0037] This embodiment relates to a method for detecting 17β-estradiol by colorimetry, which specifically includes the following steps:

[0038] (1) Preparation of a detection system consisting of 17β-estradiol aptamer: In a 1.5mL graduated centrifuge tube, add 15 μL of 17β-estradiol aptamer (sequence such as SEQ ID No. 1), add 205 μL of buffer and place it at 25°C for later use; the preparation of the buffer is as follows: weigh 0.5232 g of propanesulfonic acid and dissolve it in ultrapure water,...

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Abstract

The invention discloses a method for detecting 17beta-estradiol by employing a colorimetric method based on a nucleic acid aptamer. The method comprises the following steps: drawing a standard curve, namely preparing a 17beta-estradiol standard system, recording absorbance values of a standard solution and a contrast solution under the conditions of wavelengths of 650nm and 520nm, plotting by virtue of the specific value difference Delta A between the relative absorbance corresponding to the standard solution and the contrast solution, and drawing a standard curve; detecting a 17beta-estradiol sample, namely adding a to-be-detected sample to a buffer solution containing the nucleic acid aptamer, mixing evenly, incubating and simultaneously adding water to obtain the contrast solution; adding a cationic polymer solution, mixing evenly and incubating; adding a nano gold solution, mixing evenly and incubating; and calculating the 17beta-estradiol content in the to-be-tested sample, namely determining the concentration of the 17beta-estradiol in the to-be-tested sample according to the standard curve corresponding to the difference value Delta A between A650/A520 measured by the sample and the A650/A520 of a blank control group.

Description

technical field [0001] The invention belongs to the field of detection of 17β-estradiol in water bodies, and relates to a method for detecting 17β-estradiol based on a nucleic acid aptamer-based colorimetric method, in particular to a nucleic acid aptamer, a cationic polymer and a nano-cooperative method. A colorimetric method for the detection of 17β-estradiol. Background technique [0002] 17β-estradiol is a naturally occurring steroid hormone and is an estrogen with strong estrogenic activity in humans. It is synthesized by steroids in the human body and plays a very important role in the formation and maintenance of female sex characteristics. At the same time, 17β-estradiol is also a typical environmental endocrine disruptor. Exposure experiments show that even low concentrations of 17β-estradiol in water can disrupt the endocrine system of fish and make male fish tend to be feminized. 17β-estradiol can enter the environment through the metabolism of humans and animal...

Claims

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Application Information

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IPC IPC(8): G01N33/74
CPCG01N33/743G01N2333/575
Inventor 王鲁梅张东伟张暐霖詹深山叶嘉赟夏兵
Owner SHANGHAI JIAO TONG UNIV
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