Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer
A technology of nucleic acid aptamer and estradiol, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of complex sample pretreatment, etc., and overcome the relatively complicated electrochemical operation, simple operation, and good selectivity Effect
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[0036] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.
[0037] This embodiment relates to a method for detecting 17β-estradiol by colorimetry, which specifically includes the following steps:
[0038] (1) Preparation of a detection system consisting of 17β-estradiol aptamer: In a 1.5mL graduated centrifuge tube, add 15 μL of 17β-estradiol aptamer (sequence such as SEQ ID No. 1), add 205 μL of buffer and place it at 25°C for later use; the preparation of the buffer is as follows: weigh 0.5232 g of propanesulfonic acid and dissolve it in ultrapure water,...
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