Melanoma-resistant stem cell patch and preparation method thereof

An anti-melanoma and stem cell technology, applied in the fields of biotechnology and regenerative medicine, can solve the problems of ineffective treatment of malignant melanoma and difficult to achieve radical cure, and achieve the effects of increasing interferon synthesis, promoting apoptosis, inhibiting growth and metastasis

Active Publication Date: 2015-05-20
奥思达干细胞有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, many tumors have already metastasized when they are discovered, so it is difficult to achieve a radical cure by simple

Method used

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  • Melanoma-resistant stem cell patch and preparation method thereof
  • Melanoma-resistant stem cell patch and preparation method thereof
  • Melanoma-resistant stem cell patch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0025] Specific embodiment 1: as figure 1 Shown is a flow chart of the preparation steps of an anti-melanoma stem cell patch of the present invention, and the described method comprises the following steps:

[0026] Step 1: Preparation of bone marrow-derived mesenchymal stem cells

[0027] The femoral bone marrow of spontaneously aborted fetuses was collected under sterile conditions (human embryonic bone marrow-derived mesenchymal stem cells used in the experiment were all spontaneously aborted fetuses, and the mothers of the aborted fetuses were informed and signed the consent form agreeing that the aborted fetuses were used for stem cell research), and used 20% fetal bovine Wash the bone marrow of the femur with the DMEM medium (containing penicillin 100 / mL, streptomycin 100 μg / mL, pH7.4) and pour it into a plate, and use a syringe to suck and blow properly to make the bone marrow tissue cells basically evenly dispersed, and then count , to 1 x 10 4 / cm 2 Density inocu...

specific Embodiment 2

[0041] Specific Example 2: Flow cytometric identification of mesenchymal stem cells derived from bone marrow

[0042] The isolated human embryonic bone marrow-derived mesenchymal stem cells were detected by flow cytometry: more than 95% of CD166-positive cells and less than 3% of CD34-positive cells (CD166 is a characteristic surface marker of BMSC cell membrane, and BMSC cell membrane does not express CD34 ), the cell viability detected by trypan blue was above 95%.

Embodiment 3

[0043] Embodiment 3 animal test

[0044] 1. Preparation of melanoma mouse model

[0045] ①The preparation concentration is 1×10 7 cells / ml B16 melanoma cell suspension; ②Use a dermatome to remove the back hair of 6-8 week-old C57BL / 6J mice, and the hair removal area is about 4cm 2 ; ③ B16 melanoma cells were subcutaneously injected into the back of 40 mice, 0.2ml each; the tumor growth was observed the next day, the size of the induration was measured, the survival period of the mice was observed, and relevant data were recorded.

[0046] 2. Treatment of Melanoma Mice

[0047] One week later, the tumor-bearing mice were randomly divided into two groups, 20 in each group. The experimental group had the successfully prepared stem cell patch applied to the tumor body, and the control group had no patch applied as a blank control. The patch was replaced every four days, and the treatment continued. 4 times. When replacing the patch, measure the length and width of the tumor w...

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Abstract

The invention provides a melanoma-resistant stem cell patch. The stem cell patch has a mesenchymal stem cell molecular mark and a biological characteristic, low immunogenicity, does not cause immunological rejection, and by adding effective factors aiming at melanoma, the melanoma can be effectively treated and restored. The invention further provides a preparation method of the stem cell patch, and the preparation method comprises the following steps: preparing a mesenchymal stem cell from bone marrows, preparing and pretreating a biomembrane, constructing a mesenchymal stem cell biomembrane, inducing the mesenchymal stem cell biomembrane, and forming the melanoma-resistant stem cell patch. The method adopts stem cell products with wide sources, the preparation process is simple and reliable and the reproducibility is good.

Description

technical field [0001] The invention relates to the fields of biotechnology and regenerative medicine, in particular to an anti-melanoma stem cell patch and a preparation method thereof. Background technique [0002] Malignant melanoma is a highly malignant tumor, and its incidence rate accounts for the third place among dermatological tumors, and it is increasing year by year. Malignant melanoma has a high degree of malignancy, and often metastasizes, and the prognosis is relatively serious. Patient mortality also increases with the depth of tumor invasion. At present, there is no other effective treatment except early detection and surgical resection. But at present, many tumors have already metastasized when they are discovered, so simple surgical resection is difficult to achieve the purpose of radical cure. However, radiotherapy and chemotherapy as adjuvant methods have little effect on the treatment of malignant melanoma. Therefore, seeking an efficient and safe ad...

Claims

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Application Information

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IPC IPC(8): A61K38/20A61K9/70A61K47/42A61K47/34A61P35/00A61K31/375A61K35/16A61K35/28
Inventor 周萱
Owner 奥思达干细胞有限公司
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