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Tissue culture and rapid propagation method of Aleurites moluccana

A technology of tissue culture rapid propagation and chestnut, applied in the field of plant tissue culture, to achieve the effect of promoting the rapid development of biomass energy industry

Inactive Publication Date: 2015-06-03
朱海燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the reproduction of chestnut is mainly wild natural reproduction, and there are few reports on the rapid propagation of weaving culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Collection and disinfection of explants: Spray a mixed solution consisting of tetracycline 75mg / L + gentamicin 50mg / L + rifampicin 5mg / L + carbendazim 5g / L in the afternoon before the material collection, mix and spray evenly On the front and back of the leaves of the chestnut. In the afternoon of the next day, select healthy plants without pests and diseases. When collecting materials, first wipe the surface with 75% alcohol on the young stem tip, and then remove 3 to 4 ungerminated axillary buds from the stem tip and immediately put them in a beaker containing 1% ascorbic acid solution and bring them back to the laboratory. Rinse under running water for 2 hours, gently scrub the material with a 3% detergent solution with a soft brush, and then rinse with tap water for 60 minutes in drips. Soak in 10% sodium hypochlorite solution for 45 seconds in an ultra-clean workbench, rinse with sterile water three times, then disinfect with 0.1% mercuric chloride solution fo...

Embodiment 2

[0026](1) Collection and disinfection of explants: Spray a mixed solution consisting of tetracycline 75mg / L + gentamicin 50mg / L + rifampicin 5mg / L + carbendazim 5g / L in the afternoon before the material collection, mix and spray evenly On the front and back of the leaves of the chestnut. In the afternoon of the next day, select healthy plants without pests and diseases. When collecting materials, first wipe the surface with 75% alcohol on the young stem tip, and then remove 3 to 4 ungerminated axillary buds from the stem tip and immediately put them in a beaker containing 1% ascorbic acid solution and bring them back to the laboratory. Rinse under running water for 3 hours, gently scrub the material with a 3% washing powder solution with a soft brush, and then rinse with tap water for 90 minutes in the form of dripping water. Soak in 12% sodium hypochlorite solution for 50 seconds in an ultra-clean workbench, rinse 5 times with sterile water, then disinfect with 0.1% mercuric...

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Abstract

The invention discloses a tissue culture and rapid propagation method of Aleurites moluccana. The Aleurites moluccana is a Euphorbiaceae Aleurites woody oil plant. Currently, the Aleurites moluccana is mainly propagated wildly and naturally, propagation and seedling-raising with the tissue culture and rapid propagation method are rarely reported. The Aleurites moluccana is an often cross-pollinated plant, differences between wild seedlings are large, and propagation of seedlings with seeds is adverse to keeping of excellent properties such as the high oil content of excellent seed trees. In order to satisfy the huge demand of energy forest forestation for Aleurites moluccana seedlings, Aleurites moluccana stem segments with axillary buds are used as explants, in-vitro replantation plants of the Aleurites moluccana are obtained through processes of explant sterilization, induction culture, subculture, rooting culture, acclimatization and transplant and the like, a tissue culture and rapid propagation technical system for the Aleurites moluccana is established, and the method has the important significance for promotion of rapid development of the biomass energy industry.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of chestnut tissue culture. Background technique [0002] With the rapid development of society and economy, the consumption of fossil energy has increased sharply and the storage capacity has decreased rapidly, facing the pressure of depletion crisis, the development and application of renewable biomass energy has become a new trend of development. The discovery of energy tree species such as jatropha, oil palm, pistachio and chestnut has attracted great attention in the field of global biomass energy research, development and application, and is considered to be one of the important alternative resources to solve the global energy crisis. [0003] Chestnut ( Aleurites moluccana ) is a woody oil-bearing plant of the Euphorbiaceae Chestnut genus. The oil in the mature seed embryo can be used as an industrial oil so...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 朱海燕
Owner 朱海燕
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