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SNP molecular marker related to breast muscle weight and breast muscle percentage of chicken and application of SNP molecular marker

A molecular marker and rate-related technology, applied in the field of molecular biology, can solve the problems of subjectivity in judgment by limited personnel, unstable measurement accuracy, and large differences in judgment standards, achieving high accuracy, simple operation, and low cost Effect

Active Publication Date: 2015-06-03
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both of these methods are limited by the subjectivity of the determination personnel's judgment, and the judgment standards vary greatly among different people, requiring technicians to have rich experience accumulation. Therefore, the measurement accuracy is not stable, which is not conducive to popularization and application.

Method used

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  • SNP molecular marker related to breast muscle weight and breast muscle percentage of chicken and application of SNP molecular marker
  • SNP molecular marker related to breast muscle weight and breast muscle percentage of chicken and application of SNP molecular marker
  • SNP molecular marker related to breast muscle weight and breast muscle percentage of chicken and application of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Determination of the SNP markers associated with chicken breast muscle weight and breast muscle rate and the establishment of a method for detecting this site

[0035] 1. Genome-wide association analysis (GWAS) obtained SNP molecular markers that were significantly correlated with pectoral muscle weight and pectoral muscle rate near the GJA1 gene.

[0036] 1) Determination of experimental animals and target traits

[0037] A random resource group was established by using the Beijing oil chicken conservation population. 50 roosters and 250 hens will form 50 rooster families according to the male-to-female ratio of 1:5. After the sex identification of the chicks, only roosters were selected for breeding, and a total of 728 offspring roosters were obtained. During the feeding process, free access to food and water was used, and the diet was referred to the feeding standard for yellow-feathered broilers (NY / T33-2004).

[0038] Experimental chicken wing venous b...

Embodiment 2

[0054] Example 2 utilizes different populations to verify the validity of the marker

[0055] A population of 160 chickens was selected from the breeding line of Beijing oil chicken (the chickens with high intramuscular fat were mainly selected, and breast muscle weight and breast muscle ratio were not selected), and the ratio of male to female was 1:1. According to the method in Example 1, the breast muscle weight, whole eviscerated weight, leg muscle weight, and intramuscular fat content of the breast muscle were measured at 90 days of slaughter. At the same time, venous blood was collected to extract genomic DNA. The primers and PCR reaction in Example 1 were used to amplify, perform direct sequencing, and perform genotype typing at the GJA1_SNP T1289C locus.

[0056] Using SAS software, the genotype of this locus and related traits (chest muscle weight, chest muscle rate, intramuscular fat content) were analyzed by least square method. The dominant allele (CC type) had a...

Embodiment 3

[0060] Embodiment 3 utilizes the SNP molecular marker assisted selection of the present invention to improve the breeding method of chest muscle weight and chest muscle rate

[0061] 1. Groups to be selected

[0062] 300 chickens to be tested were randomly selected, and the ratio of male to female was 1:1. Blood was collected from the wing vein at about 20 days old, added with ACD anticoagulant, and stored at -20°C for later use.

[0063] 2. DNA extraction

[0064] Genomic DNA was extracted by conventional phenol imitation method, dissolved in TE buffer, the purity and concentration of DNA were double detected by agarose gel electrophoresis and ultraviolet spectrophotometry, and then diluted to a concentration of 50ng / μl.

[0065] 3. PCR reaction and sequence determination

[0066] The primers and PCR reaction in Example 1 were used to amplify, perform direct sequencing, and perform genotype typing at the GJA1_SNP T1289C locus. Allelic sequencing of the amplified products ...

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Abstract

The invention provides an SNP molecular marker related to the breast muscle weight and the breast muscle percentage of a chicken and application of the SNP molecular marker. The invention finds that the SNP molecular marker remarkably related to the breast muscle weight and the breast muscle percentage exists in a GJA1 gene on the basis of GWAS (Genome Wide Association Study). The invention provides a kit for detecting the SNP molecular marker; and the kit contains a pair of primers of which the nucleotide sequences are shown in SEQ ID NO. 1-2. The invention further provides a method for selecting a high-quality chicken pure line with high breast muscle weight and breast muscle percentage by utilizing the SNP site of the GJAI gene. The method comprises the following steps: obtaining a chicken genome DNA; detecting the genetype of the SNP T1289c site of the GJAI gene by virtue of the pair of primers of which the nucleotide sequences are shown in SEQ ID NO. 1-2; and selecting chicken individuals with superior genetype CC. The kit provided by the invention is simple to operate, high in flexibility, high in accuracy and low in detection cost and has an important application value.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a SNP molecular marker related to chicken breast muscle weight and breast muscle rate, a kit for detecting the molecular marker, and applications of the SNP molecular marker and the kit. Background technique [0002] Yellow feather broiler (also known as high-quality broiler) industry is a traditional industry with Chinese characteristics, which has occupied nearly half of my country's broiler production. The genetic improvement of high-quality broilers is to select and improve other performances such as meat production, which is relatively insufficient, on the premise of maintaining muscle quality. For example, local high-quality broiler chicken breeds such as Beijing oil chicken are famous for their high intramuscular fat content and excellent muscle quality traits, but they have always had problems such as underdeveloped breast muscles, low breast muscle rate, low me...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 文杰刘冉冉赵桂苹郑麦青李庆贺
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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