A method for preparing active peptide feed additives by utilizing leftovers of aquatic products

Abstract

The invention discloses a method for preparing an active peptide feed additive from aquatic product leftovers, comprising the following main steps: mincing the fresh aquatic product leftovers; adding the minced aquatic product leftovers into a fermentation tank, further adding nutrients and water, and mixing together to obtain a fermentation culture medium; sterilizing and cooling the culture medium in the fermentation tank, and then inoculating Bacillus subtilis HL-1 capable of producing subtilin to carry out liquid state aerobic fermentation; and after fermentation, heating up fermentation liquid to 90 DEG C, deactivating enzyme for 5min, then, removing sediments by filtering to obtain a filtrate, and further carrying out vacuum concentration and spray drying on the filtrate to obtain the active peptide feed additive. The active peptide feed additive prepared by the method is rich in antibacterial peptide and nutritional small peptides, has good growth promoting performance and disease resistance, and has good application foreground and social significance.

Description

technical field [0001] The invention relates to a method for preparing an active peptide feed additive by using leftovers of aquatic products, belonging to the field of processing and utilization of aquatic products. Background technique [0002] Since the 1950s, antibiotics, chemically synthesized drugs, hormones, β-stimulants, heavy metals, sedatives and other growth-promoting health care agents have been widely used in animal feeding and feed. Antibiotics and other growth-promoting health care agents have been used in feed for a long time. It has created a revolution in the breeding industry and the feed industry, making large-scale farming develop rapidly all over the world, and the production quantity and feed conversion rate of animals have been greatly improved. increase in magnitude. However, just as all achievements of industrial civilization have its negative effects, the long-term use of non-green feed additives such as antibiotics has caused the following proble...

AI Technical Summary

Problems solved by technology

However, just as all achievements of industrial civilization have its negative effects, the long-term use of non-green feed additives such as antibiotics has caused the following problems: serious drug residues in livestock and aquatic products, directly endangering human health; livestock and poultry and The quality of aquatic products has declined, lacking market competitiveness and trust; bacterial resistance has increased, and drug-resistant strains have increased, threatening human safety; destroying the ecological environment, etc.

At present, the antimicrobial peptides of microbial origin are the most researched on the antibacterial peptides of lactic acid bacteria, while the research on subtilisin, a new type of bacteriocin antibacterial peptide produced by Bacillus subtilis, is rare in the world, and even rarer in China.