Cell co-culture model for screening antiosteoporosis drugs and application of cell co-culture model

An anti-osteoporosis, co-cultivation technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problem of long cycle, defects in evaluation methods, and inability to comprehensively simulate the coupling of bone reconstruction in vivo, etc. question

CN104694468AInactive Publication Date: 2015-06-10DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
0 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2015-06-10
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of cell in-vitro culture, and relates to mesenchymal stem cells / osteoclast precursor cells / follicular cells co-culture model which is used for rapidly screening antiosteoporosis drugs. A co-culture system is characterized in that the mesenchymal stem cells and the osteoclast precursor cells are directly cultured in a contacting manner in a lower layer culture chamber in an upper and a lower layers of culture systems, the follicular cells are cultured on a semipermeable membrane of the upper layer culture chamber in an adherence manner: the culture system is used for simulating the bone remodeling process of bone tissues under an in-vitro condition; the cell co-culture model is used for rapidly screening the antiosteoporosis drugs according to the influence of the drugs on the release of alkaline phosphatase, tartrate-resistant acid phosphatase and estrogen.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of in vitro cell culture, and in particular relates to a bone marrow mesenchymal stem cell / osteoclast precursor cell / follicular granulosa cell co-culture model for screening anti-osteoporosis drugs and its application Background technique

[0002] With the aging of society, the incidence of osteoporosis is on the rise. At present, there are 200 million osteoporosis patients in the world, and there are more women than men. The elderly in China rank first in the world, and there are 90 million osteoporosis patients, accounting for 7.1% of the total population. It is estimated that by 2050, the number of osteoporotic patients worldwide will increase to 221 million. At that time, more than half of the world's osteoporotic fractures will occur in Asia, most of which will be in China. The increasingly serious incidence of osteoporosis suggests that the clinically used anti-osteoporosis drugs are not effective, so...

Examples

Embodiment 1

[0031] Material: