Cell co-culture model for screening antiosteoporosis drugs and application of cell co-culture model

Abstract

The invention belongs to the technical field of cell in-vitro culture, and relates to mesenchymal stem cells / osteoclast precursor cells / follicular cells co-culture model which is used for rapidly screening antiosteoporosis drugs. A co-culture system is characterized in that the mesenchymal stem cells and the osteoclast precursor cells are directly cultured in a contacting manner in a lower layer culture chamber in an upper and a lower layers of culture systems, the follicular cells are cultured on a semipermeable membrane of the upper layer culture chamber in an adherence manner: the culture system is used for simulating the bone remodeling process of bone tissues under an in-vitro condition; the cell co-culture model is used for rapidly screening the antiosteoporosis drugs according to the influence of the drugs on the release of alkaline phosphatase, tartrate-resistant acid phosphatase and estrogen.

Description

technical field [0001] The invention belongs to the technical field of in vitro cell culture, and in particular relates to a bone marrow mesenchymal stem cell / osteoclast precursor cell / follicular granulosa cell co-culture model for screening anti-osteoporosis drugs and its application Background technique [0002] With the aging of society, the incidence of osteoporosis is on the rise. At present, there are 200 million osteoporosis patients in the world, and there are more women than men. The elderly in China rank first in the world, and there are 90 million osteoporosis patients, accounting for 7.1% of the total population. It is estimated that by 2050, the number of osteoporotic patients worldwide will increase to 221 million. At that time, more than half of the world's osteoporotic fractures will occur in Asia, most of which will be in China. The increasingly serious incidence of osteoporosis suggests that the clinically used anti-osteoporosis drugs are not effective, so...

AI Technical Summary

Problems solved by technology

The above-mentioned in vitro model cannot comprehensively simulate the coupling of bone remodeling in vivo, and it is defective as an evaluation method for drug screening

The in vivo animal model evaluation methods for regulating bone remodeling drugs are mainly used: such as oral administration of glucocorticoids, intragastric retinoic acid induction, or bilateral ovariectomy to induce osteoporosis models, etc. Although animal models can more closely simulate bone Reconstruction of abnormal situations, but the cycle is long and expensive, and it is not suitable for rapid evaluation research in the early discovery stage of drugs. There is an urgent need to establish a new screening method in this field

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