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High spatial resolution laser confocal spectroscopy-mass spectrometry microscopy imaging method and device

A high-spatial-resolution, micro-imaging technology, applied in the direction of measuring devices, analytical materials, material excitation analysis, etc., can solve the problem of low spatial resolution of mass spectrometry detection, large laser focus spot, inability to neutralize atoms, molecules, and neutral ions and group detection to improve spatial resolution and suppress drift

Active Publication Date: 2017-08-25
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;
[0007] 2) It is impossible to detect neutral atoms, molecules, neutral ions and groups, etc., which restricts the accurate and complete acquisition of the mass spectrum components of the sample;
[0008] 3) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

Method used

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  • High spatial resolution laser confocal spectroscopy-mass spectrometry microscopy imaging method and device
  • High spatial resolution laser confocal spectroscopy-mass spectrometry microscopy imaging method and device
  • High spatial resolution laser confocal spectroscopy-mass spectrometry microscopy imaging method and device

Examples

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Embodiment 1

[0053] The embodiment of the present invention is based on Figure 4 The shown high spatial resolution laser confocal spectrum-mass spectrometer imaging device includes a laser point light source system 24, a collimator lens 25 placed along the optical axis direction, an exit beam attenuator 34, a ring light generation system 3, and a beam splitter 5 , the middle hole color separator 6 and the middle hole measurement objective lens 7 which is located in the refraction direction of the optical axis and focuses the light beam reflected by the middle hole color separator 6 to the measured sample 8, including the reflected light intensity of the focused light spot of the middle hole measurement objective lens 7 The confocal light intensity detection system consisting of the detection beam attenuator 35, the collecting lens 12, the light intensity point detector 2 located at the focal point of the laser lens 12, and the plasma for detecting the focused light spot of the middle hole ...

Embodiment 2

[0075] Such as Figure 4 As shown, in the high-spatial-resolution laser confocal mass spectrometer imaging device of Embodiment 1, the computer 10 can use the position z corresponding to the maximum value M of the confocal axial intensity curve 15 B The value is used to control the axial objective lens scanner 11 so that the focusing spot of the middle hole measurement objective lens 7 is focused on the measured sample 8, and the plasma plume 9 is generated by desorption and ionization.

Embodiment 3

[0077] Such as Figure 5As shown, in the high spatial resolution laser confocal spectrum-mass spectrometer imaging device of Embodiment 1, the ring light generation system 3 is replaced by a vector beam generation system 21 and a pupil filter 22 placed along the optical axis direction to generate a vector beam , a ring-shaped beam 23 is generated, which is reflected by the center-hole color separator 6 and focused by the center-hole measurement objective lens 7 to irradiate the measured sample 8 with a tiny spot exceeding the diffraction limit.

[0078] The remaining imaging measurement methods are the same as in Example 1.

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Abstract

The invention relates to a high-spatial-resolution laser confocal light spectrum-mass spectrum microscopic imaging method and device, and belongs to the field of a confocal microscopic imaging technique, a light spectrum imaging technique and a mass spectrum imaging technique. The method comprises the following steps: combining the confocal imaging technique, the mass spectrum imaging technique and a light spectrum detection technique; axially focusing and imaging samples by using focusing spots of a confocal microscopic system; carrying out micro area mass spectrum imaging on electrified molecules, atoms and the like which are generated by desorption and ionization of the samples by using focusing spots of the confocal microscopic system through a mass spectrum system; carrying out spectral imaging on emission light spectrum information of plasmas generated by desorption and ionization of the samples by using focusing spots of the confocal microscopic system through a light spectrum detection system; carrying out fusion treatment on detection data to realize detection of high-resolution shape and components of micro areas of the samples. By virtue of the method, the defect that the interference of focal plane stray light cannot be inhibited by the existing confocal microscopic imaging technique is overcome; the resistance to the stray light is high; a novel effective technical method is provided for the high-resolution imaging of the biomass spectrometry.

Description

technical field [0001] The invention belongs to the field of confocal microscopic imaging technology, spectral imaging technology and mass spectrometry imaging technology, combines laser confocal microscopic imaging technology, spectral imaging technology and mass spectrometry imaging technology, and relates to a high spatial resolution laser confocal spectral imaging-mass spectroscopic microscopic imaging method With the device, it can be used for high-resolution imaging of biological mass spectrometry. [0002] technical background [0003] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on multiple small areas in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/63G01N27/64
Inventor 赵维谦邱丽荣
Owner BEIJING INSTITUTE OF TECHNOLOGYGY