Method for Determining the Germination Rate of Uterospores of Wheat Stripe Rust Using Near Infrared Spectroscopy
A technology of near-infrared spectroscopy and spore germination rate, which is applied in the field of detection of fungal spore germination rate, can solve the problems of measurement and research reports, no germination rate of pathogenic spores, etc., and achieve the effect of saving time, saving pathogenic spores and low cost
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[0046] 1. Collect wheat stripe rust samples: Soak the seeds of wheat variety Mingxian 169 in water to accelerate germination for 24 hours, select seeds that germinate well and grow uniformly in small pots with a diameter of 10 cm, and sow about 20 seeds in each pot, and then place Cultivate indoors in an artificial climate with environmental parameters of 12 hours of light, light intensity of 10,000 lux, temperature of 11-13°C, and relative humidity of 60%-70%. Artificial spray inoculation of wheat stripe rust was carried out when the first leaf of the wheat seedling was fully unfolded in the one-leaf-one-heart stage. Take out the required physiological race of stripe rust bacteria from the liquid nitrogen tank where the bacteria are stored, bathe in water at 40°C for 5 minutes, then hydrate in the dark at 4°C for 12 hours, and make a spore suspension with an appropriate amount of bacteria and 0.2% Tween-80. Dip your fingers in clean water to remove the wax on the surface of t...
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