Method for determining wheat stripe rust disease uredospore germination rate by utilizing near-infrared spectroscopy

A near-infrared spectroscopy, spore germination rate technology, applied in the field of fungal spore germination rate detection, can solve the problem of no pathogen spore germination rate, measurement research reports, etc., and achieves the effect of saving pathogen spores, saving time, and high accuracy

Active Publication Date: 2015-06-24
CHINA AGRI UNIV
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Problems solved by technology

Plant diseases and plant pathogenic bacteria can be identified based on near-infrared spectroscopy. At present, ther

Method used

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  • Method for determining wheat stripe rust disease uredospore germination rate by utilizing near-infrared spectroscopy
  • Method for determining wheat stripe rust disease uredospore germination rate by utilizing near-infrared spectroscopy
  • Method for determining wheat stripe rust disease uredospore germination rate by utilizing near-infrared spectroscopy

Examples

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Embodiment 1

[0046] 1. Collect wheat stripe rust samples: Soak the seeds of wheat variety Mingxian 169 in water to accelerate germination for 24 hours, select seeds that germinate well and grow uniformly in small pots with a diameter of 10 cm, and sow about 20 seeds in each pot, and then place Cultivate indoors in an artificial climate with environmental parameters of 12 hours of light, light intensity of 10,000 lux, temperature of 11-13°C, and relative humidity of 60%-70%. Artificial spray inoculation of wheat stripe rust was carried out when the first leaf of the wheat seedling was fully unfolded in the one-leaf-one-heart stage. Take out the required physiological race of stripe rust bacteria from the liquid nitrogen tank where the bacteria are stored, bathe in water at 40°C for 5 minutes, then hydrate in the dark at 4°C for 12 hours, and make a spore suspension with an appropriate amount of bacteria and 0.2% Tween-80. Dip your fingers in clean water to remove the wax on the surface of t...

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Abstract

The invention relates to the detection of a fungal spore germination rate and particularly provides a method for determining a wheat stripe rust disease uredospore germination rate by utilizing a near-infrared spectroscopy. According to the method, on the basis of the near-infrared spectroscopy, a judgment model of the spore germination rate is established according to the germination rate of the wheat stripe rust disease uredospore and the near-infrared spectrum data of a corresponding sample, acquired by a spore germination test method, and the automatic nondestructive detection of the wheat stripe rust disease uredospore termination rate can be realized.

Description

technical field [0001] The invention relates to the detection of the germination rate of fungal spores, in particular to a method for measuring the germination rate of wheat stripe rust fungus uredia by using near-infrared spectroscopy. Background technique [0002] Wheat stripe rust is an important disease in the world. The disease occurs widely in our country, and it has become a disaster for many times, which has seriously affected the wheat production in our country. Wheat stripe rust relies on uredia spores for long-distance transmission through air currents. In my country, three endemic areas have been formed: the North China-Northwest-middle and lower reaches of the Yangtze River, the Yunnan endemic area and the Xinjiang endemic area. Wheat stripe rust is an obligate parasite, which parasitizes in the host and expands continuously, and the uredospore heap produced bursts and releases uredospores. At present, the isolation, purification, cultivation and preservation o...

Claims

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Application Information

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IPC IPC(8): G01N21/359
Inventor 王海光秦丰程培李小龙赵雅琼马占鸿赵龙莲李军会
Owner CHINA AGRI UNIV
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